ArticlePloS one2014
Hydrogen-rich saline promotes survival of retinal ganglion cells in a rat model of optic nerve crush.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- GHRHR Deficiency Enhances Retinal Ganglion Cell Survival and Visual Functions in Experimental Glaucoma by Inhibiting Ferroptosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Hydrogen-rich saline treatment modulates proteomic profiles to mitigate cataract development in a N-methyl-N-nitrosourea-induced rat model.International ophthalmology · 2026Article
- Optimized intraorbital optic nerve exposure: a translational surgical paradigm for neural regeneration in rat model.International ophthalmology · 2025Article
- Neuroplastin 65 deficiency leads to the impairment of visual function through affecting ribbon synapse in retina of mice.Frontiers in cellular neuroscience · 2025Article
- Novel Role of Molecular Hydrogen: The End of Ophthalmic Diseases?Pharmaceuticals (Basel, Switzerland) · 2023Review
- The clinical research on the effect of hydrogen-rich water on primary retinitis pigmentosa.Heliyon · 2023Article
- Inhibition of ferroptosis promotes retina ganglion cell survival in experimental optic neuropathies.Redox biology · 2022Article
- Pim-1 Protects Retinal Ganglion Cells by Enhancing Their Regenerative Ability Following Optic Nerve Crush.Experimental neurobiology · 2020Article
- Review
- Evaluating retinal ganglion cell loss and dysfunction.Experimental eye research · 2016Review
- Use of Hydrogen as a Novel Therapeutic Strategy Against Photoreceptor Degeneration in Retinitis Pigmentosa Patients.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- The potential utilizations of hydrogen as a promising therapeutic strategy against ocular diseases.Therapeutics and clinical risk management · 2016Review
- Raf-1 Kinase Inhibitory Protein (RKIP) Promotes Retinal Ganglion Cell Survival and Axonal Regeneration Following Optic Nerve Crush.Journal of molecular neuroscience : MN · 2015Article
- Generation of Natural Killer Cell Memory during Viral Infection.Journal of innate immunity · 2015Review
- Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive review of 321 original articles.Medical gas research · 2015Article
- Up-regulation of SKIP relates to retinal ganglion cells apoptosis after optic nerve crush in vivo.Journal of molecular histology · 2014Article
- Effects of hydrogen-rich saline on endotoxin-induced uveitis.Medical gas researchArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate the effect of molecular hydrogen (H2) in a rat model subjected to optic nerve crush (ONC).
methodsWe tested the hypothesis that after optic nerve crush (ONC), retinal ganglion cell (RGC) could be protected by H₂. Rats in different groups received saline or hydrogen-rich saline every day for 14 days after ONC. Retinas from animals in each group underwent measurements of hematoxylin and eosin (H&E) staining, cholera toxin beta (CTB) tracing, gamma synuclein staining, and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) staining 2 weeks post operation. Flash visual evoked potentials (FVEP) and pupillary light reflex (PLR) were then tested to evaluate the function of optic nerve. The malondialdehyde (MDA) level in retina was evaluated.
resultsH&E, gamma synuclein staining and CTB tracing showed that the survival rate of RGCs in hydrogen saline-treated group was significantly higher than that in saline-treated group. Apoptosis of RGCs assessed by TUNEL staining were less observed in hydrogen saline-treated group. The MDA level in retina of H₂ group was much lower than that in placebo group. Furthermore, animals treated with hydrogen saline showed better function of optic nerve in assessments of FVEP and PLR.
conclusionThese results demonstrated that H₂ protects RGCs and helps preserve the visual function after ONC and had a neuroprotective effect in a rat model subjected to ONC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.