ArticleActa crystallographica. Section F, Structural biology communications2014
Automation in biological crystallization.
Article in Acta crystallographica. Section F, Structural biology communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Towards routine accurate electron-density studies of biological macromolecules.Acta crystallographica. Section D, Structural biology · 2026Article
- Shoebill: an interpretable AlphaFold2-informed predictor of protein crystallization propensity using XGBoost.Briefings in bioinformatics · 2026Article
- Protein-Protein Interactions as Promising Molecular Targets for Novel Antimicrobials Aimed at Gram-Negative Bacteria.International journal of molecular sciences · 2025Review
- Automation of protein crystallization scaleup via Opentrons-2 liquid handling.SLAS technology · 2025Article
- Heterogeneous Nucleation in Protein Crystallization.Biomimetics (Basel, Switzerland) · 2023Review
- Co-crystallization with diabodies: A case study for the introduction of synthetic symmetry.Structure (London, England : 1993) · 2021Article
- Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates.Journal of applied crystallography · 2021Article
- Proline/alanine-rich sequence (PAS) polypeptides as an alternative to PEG precipitants for protein crystallization.Acta crystallographica. Section F, Structural biology communications · 2020Article
- Innovation versus practice in biological crystallization.Acta crystallographica. Section F, Structural biology communications · 2020Article
- High-Throughput Crystallization Pipeline at the Crystallography Core Facility of the Institut Pasteur.Molecules (Basel, Switzerland) · 2019Review
- Successful sample preparation for serial crystallography experiments.Journal of applied crystallography · 2019Article
- A comparative anatomy of protein crystals: lessons from the automatic processing of 56 000 samples.IUCrJ · 2019Article
- A novel microseeding method for the crystallization of membrane proteins in lipidic cubic phase.Acta crystallographica. Section F, Structural biology communications · 2016Article
- A visible-light-excited fluorescence method for imaging protein crystals without added dyes.Journal of applied crystallography · 2016Article
- Lessons from ten years of crystallization experiments at the SGC.Acta crystallographica. Section D, Structural biology · 2016Article
- Cleaning protocols for crystallization robots: preventing protease contamination.Acta crystallographica. Section F, Structural biology communications · 2015Article
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2 authors.
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Abstract
Crystallization remains the bottleneck in the crystallographic process leading from a gene to a three-dimensional model of the encoded protein or RNA. Automation of the individual steps of a crystallization experiment, from the preparation of crystallization cocktails for initial or optimization screens to the imaging of the experiments, has been the response to address this issue. Today, large high-throughput crystallization facilities, many of them open to the general user community, are capable of setting up thousands of crystallization trials per day. It is thus possible to test multiple constructs of each target for their ability to form crystals on a production-line basis. This has improved success rates and made crystallization much more convenient. High-throughput crystallization, however, cannot relieve users of the task of producing samples of high quality. Moreover, the time gained from eliminating manual preparations must now be invested in the careful evaluation of the increased number of experiments. The latter requires a sophisticated data and laboratory information-management system. A review of the current state of automation at the individual steps of crystallization with specific attention to the automation of optimization is given.
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