ArticlePloS one2014
Responses of the insulin signaling pathways in the brown adipose tissue of rats following cold exposure.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Adaptive evolution at thyroid hormone, insulin and glycerolipid pathways improved energy metabolism in high-latitude Eurasian populations.Communications biology · 2026Article
- Synergistic Intervention for Obesity: Integrating Central Appetite Regulation and Peripheral Energy Expenditure.Current obesity reports · 2026Review
- Distinct sympathetic projections to brown fat regulate thermogenesis and glucose tolerance.Nature metabolism · 2026Article
- Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum-Based Hydrogels, and Explants.Macromolecular bioscience · 2025Article
- Metabolic Effects of Brown Adipose Tissue Activity Due to Cold Exposure in Humans: A Systematic Review and Meta-Analysis of RCTs and Non-RCTs.Biomedicines · 2024Review
- Constitutive Serotonin Tone as a Modulator of Brown Adipose Tissue Thermogenesis: A Rat Study.Life (Basel, Switzerland) · 2023Article
- RNA binding motif protein 3 (RBM3) promotes protein kinase B (AKT) activation to enhance glucose metabolism and reduce apoptosis in skeletal muscle of mice under acute cold exposure.Cell stress & chaperones · 2022Article
- Relationships between Uncoupling Protein GenesGenes · 2022Article
- Integrating adipocyte insulin signaling and metabolism in the multi-omics era.Trends in biochemical sciences · 2022Review
- PET/MRI-Evaluated Activation of Brown Adipose Tissue via Cold Exposure Impacts Lipid Metabolism.Metabolites · 2022Article
- Thermal stress-related physiological, behavioral, and serum biochemical responses in indigenous pigs adapted to Eastern Himalayan region.Frontiers in veterinary science · 2022Article
- An update on brown adipose tissue and obesity intervention: Function, regulation and therapeutic implications.Frontiers in endocrinology · 2022Review
- Natural Extracts That Stimulate Adipocyte Browning and Their Underlying Mechanisms.Antioxidants (Basel, Switzerland) · 2021Review
- Signaling Pathways Regulating Thermogenesis.Frontiers in endocrinology · 2021Review
- Cold-Induced RNA-Binding Protein Promotes Glucose Metabolism and Reduces Apoptosis by Increasing AKT Phosphorylation in Mouse Skeletal Muscle Under Acute Cold Exposure.Frontiers in molecular biosciences · 2021Article
- The Role of Leptin Levels in Adaptation to Cold Climates.International journal of environmental research and public health · 2020Article
- Article
- Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice.International journal of molecular sciences · 2019Article
- Fasting enhances cold resistance in fish through stimulating lipid catabolism and autophagy.The Journal of physiology · 2019Article
- Browning of white fat: agents and implications for beige adipose tissue to type 2 diabetes.Journal of physiology and biochemistry · 2019Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
unlabelledThe insulin signaling pathway is critical for the control of blood glucose levels. Brown adipose tissue (BAT) has also been implicated as important in glucose homeostasis. The effect of short-term cold exposure on this pathway in BAT has not been explored. We evaluated the effect of 4 hours of cold exposure on the insulin pathway in the BAT of rats. Whole genomic microarray chips were used to examine the transcripts of the pathway in BAT of rats exposed to 4°C and 22°C for 4 hours. The 4 most significantly altered pathways following 4 hours of cold exposure were the insulin signaling pathway, protein kinase A, PI3K/AKT and ERK/MAPK signaling. The insulin signaling pathway was the most affected. In the documented 142 genes of the insulin pathway, 42 transcripts (29.6%) responded significantly to this cold exposure with the least false discovery rate (Benjamini-Hochberg Multiple Testing: -log10 (p-value) = 7.18). Twenty-seven genes (64%) were up-regulated, including the insulin receptor (Insr), insulin substrates 1 and 2 (Irs1 and Irs2). Fifteen transcripts (36%) were down-regulated. Multiple transcripts of the primary target and secondary effector targets for the insulin signaling were also up-regulated, including those for carbohydrate metabolism. Using western blotting, we demonstrated that the cold induced higher Irs2, Irs1, and Akt-p protein levels in the BAT than in the BAT of controls maintained at room temperature, and higher Akt-p protein level in the muscle.
conclusionthis study demonstrated that 4 hours of cold exposure stimulated the insulin signaling pathway in the BAT and muscle of overnight fasted rats. This raises the possibility that acute cold stimulation may have potential to improve glucose clearance and insulin sensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.