ArticleBiochemistry2015
Nanomechanical property maps of breast cancer cells as determined by multiharmonic atomic force microscopy reveal Syk-dependent changes in microtubule stability mediated by MAP1B.
Article in Biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Nanotechnology meets medicine: applications of atomic force microscopy in disease.Biophysical reviews · 2025Review
- Incongruous Harmonics of Vibrating Solid-Solid Interface.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- The mechanopathology of the tumor microenvironment: detection techniques, molecular mechanisms and therapeutic opportunities.Frontiers in cell and developmental biology · 2025Review
- Microtubule-binding protein MAP1B regulates interstitial axon branching of cortical neurons via the tubulin tyrosination cycle.The EMBO journal · 2024Article
- Mechanical properties of human tumour tissues and their implications for cancer development.Nature reviews. Physics · 2024Article
- Evaluation of Surface Structure and Morphological Phenomena of Caucasian Virgin Hair with Atomic Force Microscopy.Medicina (Kaunas, Lithuania) · 2024Article
- Atomic force microscopy-mediated mechanobiological profiling of complex human tissues.Biomaterials · 2023Review
- Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration.Cell death & disease · 2022Article
- Viscoelastic parameterization of human skin cells characterize material behavior at multiple timescales.Communications biology · 2022Article
- Review: Cantilever-Based Sensors for High Speed Atomic Force Microscopy.Sensors (Basel, Switzerland) · 2020Review
- Role of Bcl-2 on drug resistance in breast cancer polyploidy-induced spindle poisons.Oncology letters · 2020Article
- Structural analysis of healthy and cancerous epithelial-type breast cells by nanomechanical spectroscopy allows us to obtain peculiarities of the skeleton and junctions.Nanoscale advances · 2019Article
- Expression of Syk and MAP4 proteins in ovarian cancer.Journal of cancer research and clinical oncology · 2019Article
- Review
- Mapping heterogeneity of cellular mechanics by multi-harmonic atomic force microscopy.Nature protocols · 2018Article
- Characterization of increasing stages of invasiveness identifies stromal/cancer cell crosstalk in rat models of mesothelioma.Oncotarget · 2018Article
- Optomechanical microrheology of single adherent cancer cells.APL bioengineering · 2018Article
- Article
- [Spleen tyrosine kinase inhibits proliferation and promotes apoptosis of colorectal cancer cells in vitro via regulating Fra-1].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2017Article
- Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The Syk protein-tyrosine kinase, a well-characterized modulator of immune recognition receptor signaling, also plays important, but poorly characterized, roles in tumor progression, acting as an inhibitor of cellular motility and metastasis in highly invasive cancer cells. Multiharmonic atomic force microscopy (AFM) was used to map nanomechanical properties of live MDA-MB-231 breast cancer cells either lacking or expressing Syk. The expression of Syk dramatically altered the cellular topography, reduced cell height, increased elasticity, increased viscosity, and allowed visualization of a more substantial microtubule network. The microtubules of Syk-expressing cells were more stable to nocodazole-induced depolymerization and were more highly acetylated than those of Syk-deficient cells. Silencing of MAP1B, a major substrate for Syk in MDA-MB-231 cells, attenuated Syk-dependent microtubule stability and reversed much of the effect of Syk on cellular topography, stiffness, and viscosity. This study illustrates the use of multiharmonic AFM both to quantitatively map the local nanomechanical properties of living cells and to identify the underlying mechanisms by which these properties are modulated by signal transduction machinery.
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