ArticleWorld journal of gastroenterology2014
Novel method for extracting exosomes of hepatocellular carcinoma cells.
Article in World journal of gastroenterology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Role of extracellular vesicle-associated proteins in the progression, diagnosis, and treatment of hepatocellular carcinoma.Cell & bioscience · 2024Review
- Expression and Diagnostic Value of tRNA-Derived Fragments Secreted by Extracellular Vesicles in Hypopharyngeal Carcinoma.OncoTargets and therapy · 2021Article
- Exosomes Promote the Transition of Androgen-Dependent Prostate Cancer Cells into Androgen-Independent Manner Through Up-Regulating the Heme Oxygenase-1.International journal of nanomedicine · 2021Article
- Article
- Autophagy-dependent ferroptosis drives tumor-associated macrophage polarization via release and uptake of oncogenic KRAS protein.Autophagy · 2020Article
- Delivery of LNA-antimiR-142-3p by Mesenchymal Stem Cells-Derived Exosomes to Breast Cancer Stem Cells Reduces Tumorigenicity.Stem cell reviews and reports · 2020Article
- Embryonic stem cell-derived exosomes inhibit doxorubicin-induced TLR4-NLRP3-mediated cell death-pyroptosis.American journal of physiology. Heart and circulatory physiology · 2019Article
- Mutant p53 increases exosome-mediated transfer of miR-21-3p and miR-769-3p to promote pulmonary metastasis.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2019Article
- The functional roles of exosomes-derived long non-coding RNA in human cancer.Cancer biology & therapy · 2019Review
- Targeted LC-MS/MS Proteomics-Based Strategy To Characterize in Vitro Models Used in Drug Metabolism and Transport Studies.Analytical chemistry · 2018Article
- Exosome-mediated delivery of functionally active miRNA-142-3p inhibitor reduces tumorigenicity of breast cancer in vitro and in vivo.International journal of nanomedicine · 2018Article
- A Comparison of Traditional and Novel Methods for the Separation of Exosomes from Human Samples.BioMed research international · 2018Review
- RNA-seq reveals distinctive RNA profiles of small extracellular vesicles from different human liver cancer cell lines.Oncotarget · 2017Article
- Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum.International journal of molecular medicine · 2017Article
- The role of extracellular vesicles in mediating progression, metastasis and potential treatment of hepatocellular carcinoma.Oncotarget · 2017Review
- Systematic review of factors influencing extracellular vesicle yield from cell cultures.Cytotechnology · 2016Review
- ZD7288, a selective hyperpolarization-activated cyclic nucleotide-gated channel blocker, inhibits hippocampal synaptic plasticity.Neural regeneration research · 2016Article
- Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of annexin A2.Journal of cell science · 2016Article
- Morphological and molecular features of oral fluid-derived exosomes: oral cancer patients versus healthy individuals.Journal of cancer research and clinical oncology · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo develop a novel method for the rapid and efficient extraction of exosomes secreted by tumor cells.
methodsUnlike the traditional extraction method, the supernatants of cell cultures were concentrated, and the exosomes were isolated promptly and effectively using a novel nanomaterial called ExoQuick. Coomassie brilliant blue staining was used for protein quantification, and the morphology of the exosomes extracted by both methods was visualized by transmission electron microscopy. Exosome marker proteins were detected by Western blot analysis. Two potential hepatoma-associated proteins, tissue transglutaminase 2 (TGM2) and annexin A2, were analyzed.
resultsThe exosomes separated by the new extraction assay based on the nanomaterial were disc-shaped, intact vesicles with lipid bilayer membranes. They were approximately 30-100 nm in diameter, which is similar to the diameter of exosomes isolated by the traditional method. The protein concentration of exosomes extracted by the new method was approximately 780 μg/10(8) cells, and therefore, it was 19 times higher than that of exosomes extracted in the traditional manner. There were differences between the total proteins of Huh-7 cells and the exosomal proteins. Typical exosome proteins, such as the transmembrane protein CD63 and heat shock protein 70, were confirmed. Two potential hepatoma-associated proteins were also identified. TGM2 was first found to exist in the exosomes of human liver cancer cells, but annexin A2 was not secreted into exosomes.
conclusionThe new extraction method based on the nanomaterial is quick and efficient. The cancer-associated protein TGM2 can be secreted through an exosome-mediated non-classical secretion pathway, and it may be a valuable tumor marker.
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