ArticleThe Journal of biological chemistry2014
Cytoskeletal reorganization evoked by Rho-associated kinase- and protein kinase C-catalyzed phosphorylation of cofilin and heat shock protein 27, respectively, contributes to myogenic constriction of rat cerebral arteries.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 56 citations in OpenAlex.
- Protein kinase Cδ and pharmacomechanical coupling: Re-envisioning cerebral vascular control.The Journal of physiology · 2026Article
- Advances in Understanding Calcium Sensitization in Gastrointestinal Smooth Muscles: Pathways, Interstitial Cells, and Disease.Journal of neurogastroenterology and motility · 2026Review
- Differential effect of pulsatile pressure on the myogenic responses of small arteries.Pflugers Archiv : European journal of physiology · 2025Article
- A computational framework for quantifying blood flow dynamics across myogenically-active cerebral arterial networks.Biomechanics and modeling in mechanobiology · 2025Article
- Functional bias of contractile control in mouse resistance arteries.Scientific reports · 2024Article
- A new model for evaluating pressure-induced vascular tone in small cerebral arteries.Biomechanics and modeling in mechanobiology · 2024Article
- Focal adhesion kinase activation is involved in contractile stimulation-induced detrusor muscle contraction in mice.European journal of pharmacology · 2023Article
- Heat Shock Proteins in Tooth Development and Injury Repair.International journal of molecular sciences · 2023Review
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Myosin light chain phosphorylation exhibits a gradient across the wall of cerebellar arteries under sustained ex vivo vascular tone.Scientific reports · 2023Article
- Dual thick and thin filament linked regulation of stretch- and L-NAME-induced tone in young and senescent murine basilar artery.Frontiers in physiology · 2023Article
- Contractile apparatus in CNS capillary pericytes.Neurophotonics · 2022Review
- Cadmium-induced hypertension is associated with renal myosin light chain phosphatase inhibition via increased T697 phosphorylation and p44 mitogen-activated protein kinase levels.Hypertension research : official journal of the Japanese Society of Hypertension · 2021Article
- The Calcium Signaling Mechanisms in Arterial Smooth Muscle and Endothelial Cells.Comprehensive Physiology · 2021Article
- Pro-Resolving FPR2 Agonists Regulate NADPH Oxidase-Dependent Phosphorylation of HSP27, OSR1, and MARCKS and Activation of the Respective Upstream Kinases.Antioxidants (Basel, Switzerland) · 2021Article
- Effects of Pharmacological Inhibitors of NADPH Oxidase on Myogenic Contractility and Evoked Vasoactive Responses in Rat Resistance Arteries.Frontiers in physiology · 2021Article
- EPAC in Vascular Smooth Muscle Cells.International journal of molecular sciences · 2020Review
- GRAF3 serves as a blood volume-sensitive rheostat to control smooth muscle contractility and blood pressure.Small GTPases · 2020Article
- Phosphoproteomic analysis sheds light on intracellular signaling cascades triggered by Formyl-Peptide Receptor 2.Scientific reports · 2019Article
- Effect of Weightlessness on the 3D Structure Formation and Physiologic Function of Human Cancer Cells.BioMed research international · 2019Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Our understanding of the molecular events contributing to myogenic control of diameter in cerebral resistance arteries in response to changes in intravascular pressure, a fundamental mechanism regulating blood flow to the brain, is incomplete. Myosin light chain kinase and phosphatase activities are known to be increased and decreased, respectively, to augment phosphorylation of the 20-kDa regulatory light chain subunits (LC20) of myosin II, which permits cross-bridge cycling and force development. Here, we assessed the contribution of dynamic reorganization of the actin cytoskeleton and thin filament regulation to the myogenic response and serotonin-evoked constriction of pressurized rat middle cerebral arteries. Arterial diameter and the levels of phosphorylated LC(20), calponin, caldesmon, cofilin, and HSP27, as well as G-actin content, were determined. A decline in G-actin content was observed following pressurization from 10 mm Hg to between 40 and 120 mm Hg and in three conditions in which myogenic or agonist-evoked constriction occurred in the absence of a detectable change in LC20 phosphorylation. No changes in thin filament protein phosphorylation were evident. Pressurization reduced G-actin content and elevated the levels of cofilin and HSP27 phosphorylation. Inhibitors of Rho-associated kinase and PKC prevented the decline in G-actin; reduced cofilin and HSP27 phosphoprotein content, respectively; and blocked the myogenic response. Furthermore, phosphorylation modulators of HSP27 and cofilin induced significant changes in arterial diameter and G-actin content of myogenically active arteries. Taken together, our findings suggest that dynamic reorganization of the cytoskeleton involving increased actin polymerization in response to Rho-associated kinase and PKC signaling contributes significantly to force generation in myogenic constriction of cerebral resistance arteries.
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