ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2014
'Hit and run' serial femtosecond crystallography of a membrane kinase in the lipid cubic phase.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Solid-state NMR of membrane peptides and proteins in the lipid cubic phase.Biophysical journal · 2025Article
- Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED.Structure (London, England : 1993) · 2020Article
- Strategies for sample delivery for femtosecond crystallography.Acta crystallographica. Section D, Structural biology · 2019Review
- Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography.Nature communications · 2016Article
- Graphene-based microfluidics for serial crystallography.Lab on a chip · 2016Article
- Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.Structure (London, England : 1993) · 2016Article
- Serial femtosecond crystallography opens new avenues for Structural Biology.Protein and peptide letters · 2016Article
- In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.Acta crystallographica. Section D, Structural biology · 2016Article
- Ternary structure reveals mechanism of a membrane diacylglycerol kinase.Nature communications · 2015Article
- Ultrafast isomerization initiated by X-ray core ionization.Nature communications · 2015Article
- In meso in situ serial X-ray crystallography of soluble and membrane proteins.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Structural insights into the cubic-hexagonal phase transition kinetics of monoolein modulated by sucrose solutions.Physical chemistry chemical physics : PCCP · 2015Article
- Article
- A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.Acta crystallographica. Section F, Structural biology communications · 2015Review
- The birth of a new field.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
The lipid-based bicontinuous cubic mesophase is a nanoporous membrane mimetic with applications in areas that include medicine, personal care products, foods and the basic sciences. An application of particular note concerns it use as a medium in which to grow crystals of membrane proteins for structure determination by X-ray crystallography. At least two variations of the mesophase exist. One is the highly viscous cubic phase, which has well developed long-range order. The other so-called sponge phase is considerably more fluid and lacks long-range order. The sponge phase has recently been shown to be a convenient vehicle for delivering microcrystals of membrane proteins to an X-ray free-electron laser beam for serial femtosecond crystallography (SFX). Unfortunately, the sponge phase approach calls for large amounts of protein that are not always available in the case of membrane proteins. The cubic phase offers the advantage of requiring significantly less protein for SFX but comes with its own challenges. Here, we describe the physico-chemical bases for these challenges, solutions to them and prospects for future uses of lipidic mesophases in the SFX arena.
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