ReviewPhilosophical transactions of the Royal Society of London. Series B, Biological sciences2014
Time-resolved crystallography and protein design: signalling photoreceptors and optogenetics.
Review in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- Denoising and iterative phase recovery reveal low-occupancy populations in protein crystals.Communications biology · 2025Article
- A comparative review of time-resolved x-ray and electron scattering to probe structural dynamics.Structural dynamics (Melville, N.Y.) · 2024Review
- The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals.Current opinion in structural biology · 2019Review
- Protein ensembles link genotype to phenotype.PLoS computational biology · 2019Review
- Time-Resolved Macromolecular Crystallography at Pulsed X-ray Sources.International journal of molecular sciences · 2019Review
- Article
- XFELs for structure and dynamics in biology.IUCrJ · 2017Review
- Blue protein with red fluorescence.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Imaging enzyme kinetics at atomic resolution.IUCrJ · 2016Article
- Diffraction Techniques in Structural Biology.Current protocols in nucleic acid chemistry · 2016Review
- Allosteric effects of chromophore interaction with dimeric near-infrared fluorescent proteins engineered from bacterial phytochromes.Scientific reports · 2016Article
- Ligand and interfacial dynamics in a homodimeric hemoglobin.Structural dynamics (Melville, N.Y.) · 2016Article
- Towards time-resolved serial crystallography in a microfluidic device.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Article
- Double-focusing mixing jet for XFEL study of chemical kinetics.Journal of synchrotron radiation · 2014Article
- The birth of a new field.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Article
- Molecular Dynamics Simulations of Macromolecular Crystals.Wiley interdisciplinary reviews. Computational molecular scienceArticle
Corrections and comments
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Abstract
Time-resolved X-ray crystallography and solution scattering have been successfully conducted on proteins on time-scales down to around 100 ps, set by the duration of the hard X-ray pulses emitted by synchrotron sources. The advent of hard X-ray free-electron lasers (FELs), which emit extremely intense, very brief, coherent X-ray pulses, opens the exciting possibility of time-resolved experiments with femtosecond time resolution on macromolecular structure, in both single crystals and solution. The X-ray pulses emitted by an FEL differ greatly in many properties from those emitted by a synchrotron, in ways that at first glance make time-resolved measurements of X-ray scattering with the required accuracy extremely challenging. This opens up several questions which I consider in this brief overview. Are there likely to be chemically and biologically interesting structural changes to be revealed on the femtosecond time-scale? How shall time-resolved experiments best be designed and conducted to exploit the properties of FELs and overcome challenges that they pose? To date, fast time-resolved reactions have been initiated by a brief laser pulse, which obviously requires that the system under study be light-sensitive. Although this is true for proteins of the visual system and for signalling photoreceptors, it is not naturally the case for most interesting biological systems. To generate more biological targets for time-resolved study, can this limitation be overcome by optogenetic, chemical or other means?
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