ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2014
Transmission electron microscopy as a tool for nanocrystal characterization pre- and post-injector.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Structural basis of transcription: RNA polymerase II substrate binding and metal coordination using a free-electron laser.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Detection of Microcrystals for CryoEM.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Transcription with a laser: Radiation-damage-free diffraction of RNA Polymerase II crystals.Methods (San Diego, Calif.) · 2019Article
- Strategies for sample delivery for femtosecond crystallography.Acta crystallographica. Section D, Structural biology · 2019Review
- Article
- Assessment of microcrystal quality by transmission electron microscopy for efficient serial femtosecond crystallography.Archives of biochemistry and biophysics · 2016Review
- Serial femtosecond crystallography: A revolution in structural biology.Archives of biochemistry and biophysics · 2016Review
- Transmission electron microscopy for the evaluation and optimization of crystal growth.Acta crystallographica. Section D, Structural biology · 2016Article
- Serial femtosecond crystallography opens new avenues for Structural Biology.Protein and peptide letters · 2016Article
- Data collection strategies for time-resolved X-ray free-electron laser diffraction, and 2-color methods.Structural dynamics (Melville, N.Y.) · 2015Article
- Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Goniometer-based femtosecond crystallography with X-ray free electron lasers.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The birth of a new field.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Article
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
Recent advancements at the Linac Coherent Light Source X-ray free-electron laser (XFEL) enabling successful serial femtosecond diffraction experiments using nanometre-sized crystals (NCs) have opened up the possibility of X-ray structure determination of proteins that produce only submicrometre crystals such as many membrane proteins. Careful crystal pre-characterization including compatibility testing of the sample delivery method is essential to ensure efficient use of the limited beamtime available at XFEL sources. This work demonstrates the utility of transmission electron microscopy for detecting and evaluating NCs within the carrier solutions of liquid injectors. The diffraction quality of these crystals may be assessed by examining the crystal lattice and by calculating the fast Fourier transform of the image. Injector reservoir solutions, as well as solutions collected post-injection, were evaluated for three types of protein NCs (i) the membrane protein PTHR1, (ii) the multi-protein complex Pol II-GFP and (iii) the soluble protein lysozyme. Our results indicate that the concentration and diffraction quality of NCs, particularly those with high solvent content and sensitivity to mechanical manipulation may be affected by the delivery process.
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