ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2014
Microcrystallization techniques for serial femtosecond crystallography using photosystem II from Thermosynechococcus elongatus as a model system.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- Automated microbatch-under-oil phase diagrams to rationalize serial crystallography sample preparation.IUCrJ · 2026Article
- Preparing for successful protein crystallization experiments.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Article
- Growing Crystals for X-ray Free-Electron Laser Structural Studies of Biomolecules and Their Complexes.International journal of molecular sciences · 2023Article
- All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.Biomicrofluidics · 2023Article
- Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme.Lab on a chip · 2023Article
- Regulation of light energy conversion between linear and cyclic electron flow within photosystem II controlled by the plastoquinone/quinol redox poise.Photosynthesis research · 2023Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- Ultrafast isolated molecule imaging without crystallization.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- An Overview of Microcrystal Electron Diffraction (MicroED).Annual review of biochemistry · 2021Review
- Homogeneous batch micro-crystallization of proteins from ammonium sulfate.Acta crystallographica. Section D, Structural biology · 2021Article
- Macromolecular phasing using diffraction from multiple crystal forms.Acta crystallographica. Section A, Foundations and advances · 2021Article
- Segmented flow generator for serial crystallography at the European X-ray free electron laser.Nature communications · 2020Article
- Successful sample preparation for serial crystallography experiments.Journal of applied crystallography · 2019Article
- An outlook on using serial femtosecond crystallography in drug discovery.Expert opinion on drug discovery · 2019Review
- On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies.IUCrJ · 2019Article
- Strategies for sample delivery for femtosecond crystallography.Acta crystallographica. Section D, Structural biology · 2019Review
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6 authors.
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Abstract
Serial femtosecond crystallography (SFX) is a new emerging method, where X-ray diffraction data are collected from a fully hydrated stream of nano- or microcrystals of biomolecules in their mother liquor using high-energy, X-ray free-electron lasers. The success of SFX experiments strongly depends on the ability to grow large amounts of well-ordered nano/microcrystals of homogeneous size distribution. While methods to grow large single crystals have been extensively explored in the past, method developments to grow nano/microcrystals in sufficient amounts for SFX experiments are still in their infancy. Here, we describe and compare three methods (batch, free interface diffusion (FID) and FID centrifugation) for growth of nano/microcrystals for time-resolved SFX experiments using the large membrane protein complex photosystem II as a model system.
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