ReviewPhilosophical transactions of the Royal Society of London. Series B, Biological sciences2014
Femtosecond crystallography of membrane proteins in the lipidic cubic phase.
Review in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.Biomicrofluidics · 2023Article
- Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme.Lab on a chip · 2023Article
- Electrically stimulated droplet injector for reduced sample consumption in serial crystallography.Biophysical reports · 2022Article
- Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.Nature communications · 2022Article
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- Biophysical and functional characterization of the human TAS1R2 sweet taste receptor overexpressed in a HEK293S inducible cell line.Scientific reports · 2021Article
- Serial Crystallography for Structure-Based Drug Discovery.Trends in pharmacological sciences · 2020Review
- Electric Triggering for Enhanced Control of Droplet Generation.Analytical chemistry · 2019Article
- Transporters through the looking glass. An insight into the mechanisms of ion-coupled transport and methods that help reveal them.Journal of the Indian Institute of Science · 2018Article
- Serial Femtosecond Crystallography of G Protein-Coupled Receptors.Annual review of biophysics · 2018Review
- Supersaturation-controlled microcrystallization and visualization analysis for serial femtosecond crystallography.Scientific reports · 2018Article
- A Bright Future for Serial Femtosecond Crystallography with XFELs.Trends in biochemical sciences · 2017Review
- New approaches towards the understanding of integral membrane proteins: A structural perspective on G protein-coupled receptors.Protein science : a publication of the Protein Society · 2017Review
- Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Identifying ligands at orphan GPCRs: current status using structure-based approaches.British journal of pharmacology · 2016Review
- Graphene-based microfluidics for serial crystallography.Lab on a chip · 2016Article
- Serial femtosecond crystallography: A revolution in structural biology.Archives of biochemistry and biophysics · 2016Review
- Hitchhiking on the heptahelical highway: structure and function of 7TM receptor complexes.Nature reviews. Molecular cell biology · 2016Review
- Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.Structure (London, England : 1993) · 2016Article
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5 authors.
Funding
Abstract
Despite recent technological advances in heterologous expression, stabilization and crystallization of membrane proteins (MPs), their structural studies remain difficult and require new transformative approaches. During the past two years, crystallization in lipidic cubic phase (LCP) has started gaining a widespread acceptance, owing to the spectacular success in high-resolution structure determination of G protein-coupled receptors (GPCRs) and to the introduction of commercial instrumentation, tools and protocols. The recent appearance of X-ray free-electron lasers (XFELs) has enabled structure determination from substantially smaller crystals than previously possible with minimal effects of radiation damage, offering new exciting opportunities in structural biology. The unique properties of LCP material have been exploited to develop special protocols and devices that have established a new method of serial femtosecond crystallography of MPs in LCP (LCP-SFX). In this method, microcrystals are generated in LCP and streamed continuously inside the same media across the intersection with a pulsed XFEL beam at a flow rate that can be adjusted to minimize sample consumption. Pioneering studies that yielded the first room temperature GPCR structures, using a few hundred micrograms of purified protein, validate the LCP-SFX approach and make it attractive for structure determination of difficult-to-crystallize MPs and their complexes with interacting partners. Together with the potential of femtosecond data acquisition to interrogate unstable intermediate functional states of MPs, LCP-SFX holds promise to advance our understanding of this biomedically important class of proteins.
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