ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2014
Diffraction before destruction.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
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Who cites it
112 citing papers in PubMed.
- Perspectives for pharmaceutical screening at XFEL sources using the example of Lassa virus endonuclease.Acta crystallographica. Section D, Structural biology · 2026Article
- Radiation damage in serial femtosecond crystallography studied in hemoglobin.Acta crystallographica. Section D, Structural biology · 2026Article
- Polyethylene Terephthalate Film-Based Sample Holder for Fixed-Target Serial Femtosecond Crystallography.Materials (Basel, Switzerland) · 2026Article
- Automated serial electron diffraction: implementation inJournal of applied crystallography · 2026Article
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- Biological applications at the AQUA beamline of the EuPRAXIA@SPARC_LAB free electron laser.European biophysics journal : EBJ · 2026Article
- Serial femtosecond crystallography data processing at the global science data hub center at KISTI.Scientific reports · 2026Article
- A Bright and Dynamic Future in Structural Biology.Advances in experimental medicine and biology · 2026Review
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- Development of arrival-time diagnostic tool for X-ray pump-probe experiments at Shanghai Soft X-ray Free Electron Laser.Journal of synchrotron radiation · 2026Article
- Article
- Heavy-element damage seeding in proteins under XFEL illumination.Journal of synchrotron radiation · 2025Article
- Calculation of effective pump dose in X-ray-pump/X-ray-probe experiments.Journal of synchrotron radiation · 2025Article
- Structure Determination from Single-Molecule X-ray Scattering Images Using Stochastic Gradient Ascent.Journal of chemical theory and computation · 2025Article
- Modeling electron dynamics in silicon driven by high-intensity femtosecond x-rays.Structural dynamics (Melville, N.Y.) · 2025Article
- Observation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography.Communications chemistry · 2025Article
- Advances in microfluidic mixers for time-resolved structural biology with X-rays.Biophysical reviews · 2025Review
- Redox-State-Dependent Structural Changes within a Prokaryotic 6-4 Photolyase.Journal of the American Chemical Society · 2025Article
- Review
- Probing soft X-ray induced photoreduction of a model Mn-complex at cryogenic conditions.Journal of synchrotron radiation · 2025Article
52 more citing papers are in PubMed but not listed here.
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3 authors.
Funding
Abstract
X-ray free-electron lasers have opened up the possibility of structure determination of protein crystals at room temperature, free of radiation damage. The femtosecond-duration pulses of these sources enable diffraction signals to be collected from samples at doses of 1000 MGy or higher. The sample is vaporized by the intense pulse, but not before the scattering that gives rise to the diffraction pattern takes place. Consequently, only a single flash diffraction pattern can be recorded from a crystal, giving rise to the method of serial crystallography where tens of thousands of patterns are collected from individual crystals that flow across the beam and the patterns are indexed and aggregated into a set of structure factors. The high-dose tolerance and the many-crystal averaging approach allow data to be collected from much smaller crystals than have been examined at synchrotron radiation facilities, even from radiation-sensitive samples. Here, we review the interaction of intense femtosecond X-ray pulses with materials and discuss the implications for structure determination. We identify various dose regimes and conclude that the strongest achievable signals for a given sample are attained at the highest possible dose rates, from highest possible pulse intensities.
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