ArticleFunctional & integrative genomics2014
Increased accumulation of the cardio-cerebrovascular disease treatment drug tanshinone in Salvia miltiorrhiza hairy roots by the enzymes 3-hydroxy-3-methylglutaryl CoA reductase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase.
Article in Functional & integrative genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 116 citations in OpenAlex.
- Multi-omics elucidation of temporal patterns in tanshinone accumulation in Salvia miltiorrhiza.BMC plant biology · 2026Article
- Divergent Roles of SmHMGR2 and a Novel SmHMGR5 in Tanshinone Biosynthesis Revealed by CRISPR/Cas9-Mediated Knockout inInternational journal of molecular sciences · 2026Article
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- Anti-oxidative effect of Salvia miltiorrhiza Bunge fermented with Shiraia bambusicola Henn. against HBMC complementary medicine and therapies · 2025Article
- Comparative transcriptomic analysis and genome-wide identification provide insights into the potential role of fungal-responsive MAPK cascade genes in tanshinone accumulation inFrontiers in plant science · 2025Article
- The Untapped Potential of Hairy Root Cultures and Their Multiple Applications.International journal of molecular sciences · 2024Review
- Dual-channel hypergraph convolutional network for predicting herb-disease associations.Briefings in bioinformatics · 2024Article
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- Comparative transcriptome analysis of Veratrum maackii and Veratrum nigrum reveals multiple candidate genes involved in steroidal alkaloid biosynthesis.Scientific reports · 2023Article
- Effects of cadmium on the synthesis of active ingredients inOpen life sciences · 2023Article
- Isolation ofGenes · 2022Article
- Collection of Hairy Roots as a Basis for Fundamental and Applied Research.Molecules (Basel, Switzerland) · 2022Review
- Multilevel interactions between native and ectopic isoprenoid pathways affect global metabolism in rice.Transgenic research · 2022Article
- Molecular Cloning and Functional Analysis of 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase fromGenes · 2021Article
- Lipopolysaccharide Enhances Tanshinone Biosynthesis via a CaInternational journal of molecular sciences · 2020Article
- Application ofPlanta · 2020Article
- UPLC/MS-based untargeted metabolomics reveals the changes of metabolites profile of Salvia miltiorrhiza bunge during Sweating processing.Scientific reports · 2020Article
- Tanshinone and salvianolic acid biosynthesis are regulated byJournal of advanced research · 2020Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tanshinone is widely used for treatment of cardio-cerebrovascular diseases with increasing demand. Herein, key enzyme genes SmHMGR (3-hydroxy-3-methylglutaryl CoA reductase) and SmDXR (1-deoxy-D-xylulose 5-phosphate reductoisomerase) involved in the tanshinone biosynthetic pathway were introduced into Salvia miltiorrhiza (Sm) hairy roots to enhance tanshinone production. Over-expression of SmHMGR or SmDXR in hairy root lines can significantly enhance the yield of tanshinone. Transgenic hairy root lines co-expressing HMGR and DXR (HD lines) produced evidently higher levels of total tanshinone (TT) compared with the control and single gene transformed lines. The highest tanshinone production was observed in HD42 with the concentration of 3.25 mg g(-1) DW. Furthermore, the transgenic hairy roots showed higher antioxidant activity than control. In addition, transgenic hairy root harboring HMGR and DXR (HD42) exhibited higher tanshinone content after elicitation by yeast extract and/or Ag(+) than before. Tanshinone can be significantly enhanced to 5.858, 6.716, and 4.426 mg g(-1) DW by YE, Ag(+), and YE-Ag(+) treatment compared with non-induced HD42, respectively. The content of cryptotanshinone and dihydrotanshinone was effectively elevated upon elicitor treatments, whereas there was no obvious promotion effect for the other two compounds tanshinone I and tanshinone IIA. Our results provide a useful strategy to improve tanshinone content as well as other natural active products by combination of genetic engineering with elicitors.
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