ArticleJournal of assisted reproduction and genetics2014
Embryo catheter loading and embryo culture techniques: results of a worldwide Web-based survey.
Article in Journal of assisted reproduction and genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05924048 (Effect of Reducing the Oxygen Concentration From 5% to 2% on Intracytoplasmic Sperm Injection), which is not on this map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effect of Reducing the Oxygen Concentration From 5% to 2% on Intracytoplasmic Sperm Injection (ICSI) Outcome
Who cites it
35 citing papers in PubMed.
- Low versus high volume of culture medium during embryo transfer: a randomized clinical trial.Journal of assisted reproduction and genetics · 2018Trial
- Oxygen equilibration dynamics in assisted reproductive technology embryo culture media.Journal of assisted reproduction and genetics · 2026Article
- Impacts of Oxygen Tension on Developmental Competence of Preimplantation Embryos.Biomolecules · 2026Review
- Reactive Oxygen Species in Embryo Development: Sources, Impacts, and Implications for In Vitro Culture Systems.Life (Basel, Switzerland) · 2026Review
- When less really is more: reducing embryo manipulation to lower metabolic stress and improving long term outcomes.Reproduction, fertility, and development · 2026Review
- Culture of the Human Embryo.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Oxygen Equilibration Dynamics in Assisted Reproductive Technology Embryo Culture Media.Research square · 2025Article
- Association Between Human Embryo Culture Conditions, Cryopreservation, and the Potential Risk of Birth Defects in Children Conceived Through Assisted Reproduction Technology.Medicina (Kaunas, Lithuania) · 2025Review
- Oxygen level alters energy metabolism in bovine preimplantation embryos.Scientific reports · 2025Article
- Comparison of the developmental competence of in vitro-produced mouse embryos cultured under 5 versus 2% OJournal of assisted reproduction and genetics · 2024Article
- Enhancement of preimplantation mouse embryo development with optimized in vitro culture dish via stabilization of medium osmolarity.Clinical and experimental reproductive medicine · 2023Article
- Oxygen concentration from days 1 to 3 after insemination affects the embryo culture quality, cumulative live birth rate, and perinatal outcomes.Journal of assisted reproduction and genetics · 2023Article
- Culture conditions in the IVF laboratory: state of the ART and possible new directions.Journal of assisted reproduction and genetics · 2023Review
- Opportunities and Limits of Conventional IVF versus ICSI: It Is Time to Come off the Fence.Journal of clinical medicine · 2022Review
- Article
- [Hypoxia promotes differentiation of human induced pluripotent stem cells into embryoid bodiesNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2022Article
- The effect of embryo catheter loading technique on pregnancy rate.Reproduction & fertility · 2022Article
- Update on the Epigenomic Implication of Embryo Cryopreservation Methods Applied in Assisted Reproductive Technologies With Potential Long-Term Health Effects.Frontiers in cell and developmental biology · 2022Review
- Protective Effect of Minocycline Hydrochloride on the Mouse Embryonic Development Against Suboptimal Environment.Frontiers in cell and developmental biology · 2022Article
- Biphasic (5-2%) oxygen concentration strategy significantly improves the usable blastocyst and cumulative live birth rates in in vitro fertilization.Scientific reports · 2021Observational
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo identify trends in embryo catheter loading and embryo culture techniques performed worldwide.
methodsA retrospective evaluation using the results of a web-based survey, (IVF Worldwide ( www.IVF-worldwide.com ), was performed.
resultsResponses from 265 centers in 71 countries were obtained. Most centers (97 %) preferred a catheter with its orifice on top, with only 3 % preferring a catheter with the orifice on its side; 41 % preferred a catheter marked for clear ultrasound view. The most commonly-reported methods of embryo loading were medium-air-embryo-air-medium (42 %), medium in catheter with embryo at end (20 %) and medium-air-embryo (15 %). In 68 % of centers the final volume of the catheter was up to 0.3 ml, with only 19 % using 0.3-0.5 ml and 1 % using 0.5-0.7 ml. Using reduced oxygen concentrations for embryo culture was divided between those who used it in combination with the two-gas system (34 %) and those who did not use it at all (39 %); 24 % reported using a three-gas system. Most clinics using reduced oxygen concentrations used it throughout the entire culture period. Half of centers (51 %) reported using reduced oxygen concentrations for the entire IVF population while 6 % reserved it only for blastocyst transfer. The use of sequential media was highly dominant with 40 % reporting its use.
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