ArticleBMC biology2014
ABCC transporters mediate insect resistance to multiple Bt toxins revealed by bulk segregant analysis.
Article in BMC biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 179 citations in OpenAlex.
- Interactions between insecticidal cry toxins and their receptors.Current genetics · 2025Review
- Potential Mechanisms Underlying the Minimal Impact of Cry1Ab1 Protein onInternational journal of molecular sciences · 2025Article
- Article
- Insights into diversity, host range, and evolution of iflaviruses in Lepidoptera through transcriptome mining.Virus evolution · 2025Article
- Knockdown of an ATP-binding cassette transporter in resistant western corn rootworm larvae partially reverses resistance to eCry3.1Ab protein.Scientific reports · 2024Article
- Mismatch between lab-generated and field-evolved resistance to transgenic Bt crops inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Downregulation of APN1 andApplied and environmental microbiology · 2024Article
- Utilization of Diverse Molecules as Receptors by Cry Toxin and the Promiscuous Nature of Receptor-Binding Sites Which Accounts for the Diversity.Biomolecules · 2024Review
- New Paralogs of theBiomolecules · 2024Article
- Determination of internal controls for quantitative gene expression of Spodoptera litura under microbial pesticide stress.Scientific reports · 2024Article
- Cry Toxins Use Multiple ATP-Binding Cassette Transporter Subfamily C Members as Low-Efficiency Receptors inBiomolecules · 2024Article
- ABC transporters linked to multiple herbicide resistance in blackgrass (Frontiers in plant science · 2023Article
- Bulk segregant linkage mapping for rodent and human malaria parasites.Parasitology international · 2022Review
- Molecular and Kinetic Models for Pore Formation ofToxins · 2022Article
- Optimizing bulk segregant analysis of drug resistance usingiScience · 2022Article
- Bacillus thuringiensis Cry1Ac Protoxin and Activated Toxin Exert Differential Toxicity Due to a Synergistic Interplay of Cadherin with ABCC Transporters in the Cotton Bollworm.Applied and environmental microbiology · 2022Article
- Article
- A versatile contribution of both aminopeptidases N and ABC transporters to Bt Cry1Ac toxicity in the diamondback moth.BMC biology · 2022Article
- Role of Lectin in the Response ofFrontiers in immunology · 2022Review
- Resistance in the GenusInsects · 2021Review
6 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRelatively recent evidence indicates that ABCC2 transporters play a main role in the mode of action of Bacillus thuringiensis (Bt) Cry1A-type proteins. Mapping of major Cry1A resistance genes has linked resistance to the ABCC2 locus in Heliothis virescens, Plutella xylostella, Trichoplusia ni and Bombyx mori, and mutations in this gene have been found in three of these Bt-resistant strains.
resultsWe have used a colony of Spodoptera exigua (Xen-R) highly resistant to a Bt commercial bioinsecticide to identify regions in the S. exigua genome containing loci for major resistance genes by using bulk segregant analysis (BSA). Results reveal a region containing three genes from the ABCC family (ABBC1, ABBC2 and ABBC3) and a mutation in one of them (ABBC2) as responsible for the resistance of S. exigua to the Bt commercial product and to its key Spodoptera-active ingredients, Cry1Ca. In contrast to all previously described mutations in ABCC2 genes that directly or indirectly affect the extracellular domains of the membrane protein, the ABCC2 mutation found in S. exigua affects an intracellular domain involved in ATP binding. Functional analyses of ABBC2 and ABBC3 support the role of both proteins in the mode of action of Bt toxins in S. exigua. Partial silencing of these genes with dsRNA decreased the susceptibility of wild type larvae to both Cry1Ac and Cry1Ca. In addition, reduction of ABBC2 and ABBC3 expression negatively affected some fitness components and induced up-regulation of arylphorin and repat5, genes that respond to Bt intoxication and that are found constitutively up-regulated in the Xen-R strain.
conclusionsThe current results show the involvement of different members of the ABCC family in the mode of action of B. thuringiensis proteins and expand the role of the ABCC2 transporter in B. thuringiensis resistance beyond the Cry1A family of proteins to include Cry1Ca.
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