ArticleProceedings of the National Academy of Sciences of the United States of America2014
PipX, the coactivator of NtcA, is a global regulator in cyanobacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 88 citations in OpenAlex.
- Conversion of COMicrobial cell factories · 2026Article
- From pond to platform: howJournal of bacteriology · 2026Review
- Mutational analyses reveal PLP-independent functions at PipY, the cyanobacterial paradigm for pyridoxal-phosphate binding proteins.Scientific reports · 2026Article
- Spatiotemporal 4D Whole-cell Modeling of a Minimal Autotroph Reveals Central Carbon Metabolism Regulated Locally by Protein Megacomplexes via Post-translational ModiBications under Light Disturbance.bioRxiv : the preprint server for biology · 2026Article
- Structures of the cyanobacterial nitrogen regulators NtcA and PipX complexed to DNA shed light on DNA binding by NtcA and implicate PipX in the recruitment of RNA polymerase.Nucleic acids research · 2025Article
- Temperature changes are signaled in cyanobacteria through the PipX interaction network.Frontiers in microbiology · 2025Article
- The cAMP receptor protein from Gardnerella vaginalis is not regulated by ligands.Communications biology · 2024Article
- Machine learning reveals the transcriptional regulatory network and circadian dynamics ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Role of FtsH Complexes in the Response to Abiotic Stress in Cyanobacteria.Plant & cell physiology · 2024Review
- Studies on the PII-PipX-NtcA Regulatory Axis of Cyanobacteria Provide Novel Insights into the Advantages and Limitations of Two-Hybrid Systems for Protein Interactions.International journal of molecular sciences · 2024Article
- Analysing the Cyanobacterial PipX Interaction Network Using NanoBiT Complementation inInternational journal of molecular sciences · 2024Article
- Protein NirP1 regulates nitrite reductase and nitrite excretion in cyanobacteria.Nature communications · 2024Article
- The primary carbon metabolism in cyanobacteria and its regulation.Frontiers in plant science · 2024Review
- ppGpp accumulation reduces the expression of the global nitrogen homeostasis-modulating NtcA regulon by affecting 2-oxoglutarate levels.Communications biology · 2023Article
- The Signal Transduction Protein PII Controls the Levels of the Cyanobacterial Protein PipX.Microorganisms · 2023Article
- The ribosome assembly GTPase EngA is involved in redox signaling in cyanobacteria.Frontiers in microbiology · 2023Article
- Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacteriumFrontiers in microbiology · 2023Article
- Nutrient-Limited Operational Strategies for the Microbial Production of Biochemicals.Microorganisms · 2022Review
- The Conserved Family of the Pyridoxal Phosphate-Binding Protein (PLPBP) and Its Cyanobacterial Paradigm PipY.Life (Basel, Switzerland) · 2022Review
- A mechanistic study of the influence of nitrogen and energy availability on the NH4+ sensitivity of nitrogen assimilation in Synechococcus.Journal of experimental botany · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
To modulate the expression of genes involved in nitrogen assimilation, the cyanobacterial PII-interacting protein X (PipX) interacts with the global transcriptional regulator NtcA and the signal transduction protein PII, a protein found in all three domains of life as an integrator of signals of the nitrogen and carbon balance. PipX can form alternate complexes with NtcA and PII, and these interactions are stimulated and inhibited, respectively, by 2-oxoglutarate, providing a mechanistic link between PII signaling and NtcA-regulated gene expression. Here, we demonstrate that PipX is involved in a much wider interaction network. The effect of pipX alleles on transcript levels was studied by RNA sequencing of S. elongatus strains grown in the presence of either nitrate or ammonium, followed by multivariate analyses of relevant mutant/control comparisons. As a result of this process, 222 genes were classified into six coherent groups of differentially regulated genes, two of which, containing either NtcA-activated or NtcA-repressed genes, provided further insights into the function of NtcA-PipX complexes. The remaining four groups suggest the involvement of PipX in at least three NtcA-independent regulatory pathways. Our results pave the way to uncover new regulatory interactions and mechanisms in the control of gene expression in cyanobacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.