ArticlePloS one2014
Chloroplast transformation of Platymonas (Tetraselmis) subcordiformis with the bar gene as selectable marker.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 53 citations in OpenAlex.
- Application of antimicrobial peptides expressed by Tetraselmis subcordiformis (Wille) butcher improves the health of turbot via oral administration.World journal of microbiology & biotechnology · 2026Article
- The complete chloroplast genome ofComparative cytogenetics · 2024Article
- Chloroplast Engineering: Fundamental Insights and Its Application in Amelioration of Environmental Stress.Applied biochemistry and biotechnology · 2023Review
- Harnessing the Algal Chloroplast for Heterologous Protein Production.Microorganisms · 2022Review
- Gene introduction approaches in chloroplast transformation and its applications.Journal, genetic engineering & biotechnology · 2021Review
- Recombinant Expression of Thrombolytic Agent Reteplase in Marine MicroalgaMarine drugs · 2021Article
- The Algal Chloroplast as a Testbed for Synthetic Biology Designs Aimed at Radically Rewiring Plant Metabolism.Frontiers in plant science · 2021Review
- Characterization of the Chloroplast Genome Facilitated the Transformation ofCurrent genomics · 2020Article
- Complete Chloroplast Genome Characterization ofPlants (Basel, Switzerland) · 2020Article
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- Comprehensive comparative analysis of chloroplast genomes from sevenJournal of ginseng research · 2020Article
- Review
- Engineering the Chloroplast Genome of Oleaginous Marine MicroalgaFrontiers in plant science · 2018Article
- Enhanced green fluorescent protein (egfp) gene expression in Tetraselmis subcordiformis chloroplast with endogenous regulators.World journal of microbiology & biotechnology · 2016Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this research was to establish a chloroplast transformation technique for Platymonas (Tetraselmis) subcordiformis. Employing the gfp gene as a reporter and the bar gene as a selectable marker, transformation vectors of P. subcordiformis chloroplast were constructed with endogenous fragments rrn16S-trnI (left) and trnA-rrn23S (right) as a recombination site of the chloroplast genome. The plasmids were transferred into P. subcordiformis via particle bombardment. Confocal laser scanning microscopy indicated that the green fluorescence protein was localized in the chloroplast of P. subcordiformis, confirming the activity of the Chlamydomonas reinhardtii promoter. Cells transformed with the bar gene were selected using the herbicide Basta. Resistant colonies were analyzed by PCR and Southern blotting, and the results indicated that the bar gene was successfully integrated into the chloroplast genome via homologous recombination. The technique will improve genetic engineering of this alga.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.