Evidence map›Paper›PMID 24911610›Full record

ArticlePloS one2014

microRNA expression in peripheral blood cells following acute ischemic stroke and their predicted gene targets.

Glen C Jickling, Bradley P Ander, Xinhua Zhan, Dylan Noblett, Boryana Stamova, Dazhi Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
103citing papers in PubMed, 3 pooled it
6.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

103 citing papers in PubMed, 3 syntheses or guidelines pooled it, 184 citations in OpenAlex.

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43 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Glen C JicklingDepartment of Neurology and the MIND Institute, University of California at Davis, Sacramento, California, United States of America.
Bradley P AnderDepartment of Neurology and the MIND Institute, University of California at Davis, Sacramento, California, United States of America.
Xinhua ZhanDepartment of Neurology and the MIND Institute, University of California at Davis, Sacramento, California, United States of America.
Dylan NoblettDepartment of Neurology and the MIND Institute, University of California at Davis, Sacramento, California, United States of America.
Boryana StamovaDepartment of Neurology and the MIND Institute, University of California at Davis, Sacramento, California, United States of America.
Dazhi LiuDepartment of Neurology and the MIND Institute, University of California at Davis, Sacramento, California, United States of America.
University of California, Davis · US

Funding

Molecular Biology of Stroke in HumansR01NS075035 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R · 2012 to 2016
$3.1M
Whole Genome RNA Sequencing (RNAseq) of Blood from Patients with Lacunar StrokeR01NS101718 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R, STAMOVA, BORYANA · 2017 to 2021
$3.0M
A Novel Approach to Assessing Cryptogenic StrokeR01NS079153 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHARP, FRANK R · 2012 to 2016
$2.8M
NINDS NIH HHS NS075035NINDS NIH HHS NS079153NINDS NIH HHS R01 NS075035NINDS NIH HHS R01 NS079153NINDS NIH HHS R01 NS101718
6 · The paper itself

Abstract

backgroundmicroRNA (miRNA) are important regulators of gene expression. In patients with ischemic stroke we have previously shown that differences in immune cell gene expression are present. In this study we sought to determine the miRNA that are differentially expressed in peripheral blood cells of patients with acute ischemic stroke and thus may regulate immune cell gene expression.

methodsmiRNA from peripheral blood cells of forty-eight patients with ischemic stroke and vascular risk factor controls were compared. Differentially expressed miRNA in patients with ischemic stroke were determined by microarray with qRT-PCR confirmation. The gene targets and pathways associated with ischemic stroke that may be regulated by the identified miRNA were characterized.

resultsIn patients with acute ischemic stroke, miR-122, miR-148a, let-7i, miR-19a, miR-320d, miR-4429 were decreased and miR-363, miR-487b were increased compared to vascular risk factor controls. These miRNA are predicted to regulate several genes in pathways previously identified by gene expression analyses, including toll-like receptor signaling, NF-κβ signaling, leukocyte extravasation signaling, and the prothrombin activation pathway.

conclusionsSeveral miRNA are differentially expressed in blood cells of patients with acute ischemic stroke. These miRNA may regulate leukocyte gene expression in ischemic stroke including pathways involved in immune activation, leukocyte extravasation and thrombosis.

Indexed as

Gene Expression RegulationRNA InterferenceAgedBlood CellsCase-Control StudiesComorbidityFemaleGene Regulatory NetworksHumansMaleMicroRNAsMiddle AgedNF-kappa BRisk FactorsRNA, MessengerSignal TransductionMicroRNAsNF-kappa BRNA, MessengerToll-Like Receptors

Identifiers

PMID24911610
PMCPMC4050059
OpenAlexW1967554497

What OpenQuestion holds

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LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.