ArticleAnalytical chemistry2014
Determination of rate constants and equilibrium constants for solution-phase drug-protein interactions by ultrafast affinity extraction.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 62 citations in OpenAlex.
- Studies and analysis of drug-target interactions by affinity chromatography and related techniques: A review.Journal of pharmaceutical analysis · 2026Review
- Microscale Affinity Chromatography for Biointeraction Analysis: Strategies, Principles and Applications.Journal of separation science · 2026Review
- On the Arrival Time Distribution of Reacting Systems in Ion Mobility Spectrometry.Analytical chemistry · 2024Article
- Simple Determination of Affinity Constants of Antibodies by Competitive Immunoassays.Methods and protocols · 2024Article
- Design and Biological Evaluation of Small-Molecule PET-Tracers for Imaging of Programmed Death Ligand 1.Cancers · 2023Article
- Physiologically Based Pharmacokinetic Model of Brain Delivery of Plasma Protein Bound Drugs.Pharmaceutical research · 2023Article
- Analysis of the binding of warfarin to glyoxal- and methylglyoxal-modified human serum albumin by ultrafast affinity extraction.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2022Article
- Evaluation of microcolumn stability in ultrafast affinity extraction for binding and rate studies.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2021Article
- [Advances in chromatography in the study of drug-plasma protein interactions].Se pu = Chinese journal of chromatography · 2021Review
- Characterization of drug binding with alphaJournal of chromatography. A · 2021Article
- Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods.Advances in chromatography · 2021Article
- Affinity chromatography: A review of trends and developments over the past 50 years.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2020Review
- Article
- SAR Studies and Biological Characterization of a Chromen-4-one Derivative as an Anti-ACS medicinal chemistry letters · 2019Article
- Testosterone meets albumin - the molecular mechanism of sex hormone transport by serum albumins.Chemical science · 2019Article
- Kinetic Analysis by Affinity Chromatography.Frontiers in chemistry · 2019Review
- Binding studies based on ultrafast affinity extraction and single- or two-column systems: Interactions of second- and third-generation sulfonylurea drugs with normal or glycated human serum albumin.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2018Article
- Characterization of solution-phase drug-protein interactions by ultrafast affinity extraction.Methods (San Diego, Calif.) · 2018Article
- Mobile Affinity Sorbent Chromatography.Analytical chemistry · 2018Article
- High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents.The Analyst · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A method was created on the basis of ultrafast affinity extraction to determine both the dissociation rate constants and equilibrium constants for drug-protein interactions in solution. Human serum albumin (HSA), an important binding agent for many drugs in blood, was used as both a model soluble protein and as an immobilized binding agent in affinity microcolumns for the analysis of free drug fractions. Several drugs were examined that are known to bind to HSA. Various conditions to optimize in the use of ultrafast affinity extraction for equilibrium and kinetic studies were considered, and several approaches for these measurements were examined. The dissociation rate constants obtained for soluble HSA with each drug gave good agreement with previous rate constants reported for the same drugs or other solutes with comparable affinities for HSA. The equilibrium constants that were determined also showed good agreement with the literature. The results demonstrated that ultrafast affinity extraction could be used as a rapid approach to provide information on both the kinetics and thermodynamics of a drug-protein interaction in solution. This approach could be extended to other systems and should be valuable for high-throughput drug screening or biointeraction studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.