ArticlePloS one2014
Identification and characterization of a ginsenoside-transforming β-glucosidase from Pseudonocardia sp. Gsoil 1536 and its application for enhanced production of minor ginsenoside Rg2(S).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Ginsenoside Rg2 fromMolecules (Basel, Switzerland) · 2026Review
- Genomic and metabolomic insights into the enhancement of rare ginsenosides inFrontiers in microbiology · 2026Article
- Rare ginsenosides: A unique perspective of ginseng research.Journal of advanced research · 2024Review
- American Ginseng for the Treatment of Alzheimer's Disease: A Review.Molecules (Basel, Switzerland) · 2023Review
- β-Glucosidase and Its Application in Bioconversion of Ginsenosides inBioengineering (Basel, Switzerland) · 2023Review
- Progress in the Conversion of Ginsenoside Rb1 into Minor Ginsenosides Using β-Glucosidases.Foods (Basel, Switzerland) · 2023Review
- Stem-leaves of Panax as a rich and sustainable source of less-polar ginsenosides: comparison of ginsenosides fromJournal of ginseng research · 2021Article
- Novel enzymatic elimination method for the chromatographic purification of ginsenoside RbJournal of ginseng research · 2020Article
- Attenuation of Nrf2/Keap1/ARE in Alzheimer's Disease by Plant Secondary Metabolites: A Mechanistic Review.Molecules (Basel, Switzerland) · 2020Review
- Biocatalytic strategies for the production of ginsenosides using glycosidase: current state and perspectives.Applied microbiology and biotechnology · 2020Review
- Characterization of a Novel Ginsenoside MT1 Produced by an Enzymatic Transrhamnosylation of Protopanaxatriol-Type Ginsenosides Re.Biomolecules · 2020Article
- Enzymatic Biotransformation of Ginsenoside RbJournal of microbiology and biotechnology · 2020Article
- Genotoxicity and subchronic toxicological study of a novel ginsenoside derivative 25-OCHJournal of ginseng research · 2019Article
- High-density immobilization of a ginsenoside-transforming β-glucosidase for enhanced food-grade production of minor ginsenosides.Applied microbiology and biotechnology · 2019Article
- Gypenoside LXXV Promotes Cutaneous Wound Healing In Vivo by Enhancing Connective Tissue Growth Factor Levels Via the Glucocorticoid Receptor Pathway.Molecules (Basel, Switzerland) · 2019Article
- Stereoisomers of Saponins inFrontiers in pharmacology · 2018Review
- Enhanced Production of Gypenoside LXXV Using a Novel Ginsenoside-Transforming β-Glucosidase from Ginseng-Cultivating Soil Bacteria and Its Anti-Cancer Property.Molecules (Basel, Switzerland) · 2017Article
- Improved antimicrobial effect of ginseng extract by heat transformation.Journal of ginseng research · 2017Article
- Article
- Niabella ginsenosidivorans sp. nov., isolated from compost.Journal of microbiology (Seoul, Korea) · 2015Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ginsenoside Rg2(S), which is one of the pharmaceutical components of ginseng, is known to have neuroprotective, anti-inflammation, and anti-diabetic effects. However, the usage of ginsenoside Rg2(S) is restricted owing to the small amounts found in white and red ginseng. To enhance the production of ginsenoside Rg2(S) as a 100 gram unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the recombinant glycoside hydrolase (BglPC28), which is a ginsenoside-transforming recombinant β-glucosidase from Pseudonocardia sp. strain Gsoil 1536. The gene, termed bglPC28, encoding β-glucosidase (BglPC28) belonging to the glycoside hydrolase family 3 was cloned. bglPC28 consists of 2,232 bp (743 amino acid residues) with a predicted molecular mass of 78,975 Da. This enzyme was overexpressed in Escherichia coli BL21(DE3) using a GST-fused pGEX 4T-1 vector system. The optimum conditions of the recombinant BglPC28 were pH 7.0 and 37 °C. BglPC28 can effectively transform the ginsenoside Re to Rg2(S); the Km values of PNPG and Re were 6.36 ± 1.10 and 1.42 ± 0.13 mM, respectively, and the Vmax values were 40.0 ± 2.55 and 5.62 ± 0.21 µmol min-1 mg-1 of protein, respectively. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 7.0 and 30°C for 12 hours with a concentration of 20 mg/ml of ginsenoside Re from American ginseng roots. Finally, 113 g of Rg2(S) was produced from 150 g of Re with 84.0 ± 1.1% chromatographic purity. These results suggest that this enzymatic method could be usefully exploited in the preparation of ginsenoside Rg2(S) in the cosmetics, functional food, and pharmaceutical industries.
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