ArticlePloS one2014
Three dimensional fluorescence imaging using multiple light-sheet microscopy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 41 citations in OpenAlex.
- Multicolor iLIFE (m-iLIFE) volume cytometry for high-throughput imaging of multiple organelles.Scientific reports · 2024Article
- Lightsheet optical tweezer (LOT) for optical manipulation of microscopic particles and live cells.Scientific reports · 2022Article
- Fluorescence based rapid optical volume screening system (OVSS) for interrogating multicellular organisms.Scientific reports · 2021Article
- Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array.Light, science & applications · 2020Article
- Article
- How to define and optimize axial resolution in light-sheet microscopy: a simulation-based approach.Biomedical optics express · 2020Article
- From 2D to 3D: Promising Advances in Imaging Lung Structure.Frontiers in medicine · 2020Article
- 3D Protein Dynamics in the Cell Nucleus.Biophysical journal · 2017Article
- Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes.Scientific reports · 2016Article
- A review of snapshot multidimensional optical imaging: measuring photon tags in parallel.Physics reports · 2016Article
- Single-cell phenomics in budding yeast.Molecular biology of the cell · 2015Review
- Temporal resolution in fluorescence imaging.Frontiers in molecular biosciences · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system and is capable of 3D volume imaging by simultaneously illuminating multiple planes in the sample. Theta detection geometry is employed for data acquisition from multiple specimen layers. This detection scheme inherits many advantages including, background reduction, cross-talk free fluorescence detection and high-resolution at long working distance. Using this technique, we generated 5 equi-intense light-sheets of thickness approximately 7.5 μm with an inter-sheet separation of 15 μm. Moreover, the light-sheets generated by MLSM is found to be 2 times thinner than the state-of-art SPIM system. Imaging of fluorescently coated yeast cells of size 4 ± 1 μm (encaged in Agarose gel-matrix) is achieved. Proposed imaging technique may accelerate the field of fluorescence microscopy, cell biology and biophotonics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.