ArticleHearing research2014
Primary culture of human Schwann and schwannoma cells: improved and simplified protocol.
Article in Hearing research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Article
- Article
- Reprogramming of skin fibroblasts by 3D spheroid culture promotes peripheral nerve regeneration via the ID3/semaphorin7a pathway.Stem cells translational medicine · 2025Article
- Human Schwann Cells in vitro II. Passaging, Purification, Banking, and Labeling of Established Cultures.Bio-protocol · 2023Article
- Human Schwann Cells in vitro I. Nerve Tissue Processing, Pre-degeneration, Isolation, and Culturing of Primary Cells.Bio-protocol · 2023Article
- Article
- Dedifferentiation of human epidermal melanocytes in vitro by long-term trypsinization.Cell and tissue banking · 2021Article
- Review
- Combination therapy with mTOR kinase inhibitor and dasatinib as a novel therapeutic strategy for vestibular schwannoma.Scientific reports · 2020Article
- Application of stem cells in peripheral nerve regeneration.Burns & trauma · 2020Review
- MMP-14 (MT1-MMP) Is a Biomarker of Surgical Outcome and a Potential Mediator of Hearing Loss in Patients With Vestibular Schwannomas.Frontiers in cellular neuroscience · 2020Article
- Automated image analysis of stained cytospins to quantify Schwann cell purity and proliferation.PloS one · 2020Article
- Proteomic screening identifies PML/p53 axis as a potential treatment target of facial nerve schwannomas.American journal of translational research · 2020Article
- Computational repositioning and preclinical validation of mifepristone for human vestibular schwannoma.Scientific reports · 2018Article
- Tumor-Penetrating Delivery of siRNA against TNFα to Human Vestibular Schwannomas.Scientific reports · 2017Article
- A Unified Methodological Framework for Vestibular Schwannoma Research.Journal of visualized experiments : JoVE · 2017Article
- Sulforaphane, a natural component of broccoli, inhibits vestibular schwannoma growth in vitro and in vivo.Scientific reports · 2016Article
- Extracellular vesicles derived from human vestibular schwannomas associated with poor hearing damage cochlear cells.Neuro-oncology · 2016Article
- Advances and Future Applications of Augmented Peripheral Nerve Regeneration.International journal of molecular sciences · 2016Review
- A novel alternative splicing isoform of NF2 identified in human Schwann cells.Oncology letters · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Primary culture of human Schwann cells (SCs) and vestibular schwannoma (VS) cells are invaluable tools to investigate SC physiology and VS pathobiology, and to devise effective pharmacotherapies against VS, which are sorely needed. However, existing culture protocols, in aiming to create robust, pure cultures, employ methods that can lead to loss of biological characteristics of the original cells, potentially resulting in misleading biological findings. We have developed a minimally manipulative method to culture primary human SC and VS cells, without the use of selective mitogens, toxins, or time-consuming and potentially transformative laboratory techniques. Schwann cell purity was quantified longitudinally using S100 staining in SC cultures derived from the great auricular nerve and VS cultures followed for 7 and 12 weeks, respectively. SC cultures retained approximately ≥85% purity for 2 weeks. VS cultures retained approximately ≥80% purity for the majority of the span of 12 weeks, with maximal purity of 87% at 2 weeks. The VS cultures showed high level of biological similarity (68% on average) to their respective parent tumors, as assessed using a protein array featuring 41 growth factors and receptors. Apoptosis rate in vitro negatively correlated with tumor volume. Our results, obtained using a faster, simplified culturing method than previously utilized, indicate that highly pure, primary human SC and VS cultures can be established with minimal manipulation, reaching maximal purity at 2 weeks of culture. The VS cultures recapitulate the parent tumors' biology to a great degree, making them relevant models to investigate VS pathobiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.