ArticleMolecular cell2014
Directed network wiring identifies a key protein interaction in embryonic stem cell differentiation.
Article in Molecular cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Synthetic signaling platform uncovers and rewires cellular responses to PD-1 perturbation.bioRxiv : the preprint server for biology · 2025Article
- Binding mode-guided development of high-performance antibodies targeting site-specific posttranslational modifications.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Development in Detection Methods for the Expression of Surface-Displayed Proteins.Frontiers in microbiology · 2022Article
- FGF4, A New Potential Regulator in Gestational Diabetes Mellitus.Frontiers in pharmacology · 2022Article
- Xeno- and Feeder-Free Differentiation of Human iPSCs to Trabecular Meshwork-Like Cells by Recombinant Cytokines.Translational vision science & technology · 2021Article
- Targeting Phosphotyrosine in Native Proteins with Conditional, Bispecific Antibody Traps.Journal of the American Chemical Society · 2020Article
- Surface Loops in a Single SH2 Domain Are Capable of Encoding the Spectrum of Specificity of the SH2 Family.Molecular & cellular proteomics : MCP · 2019Article
- An injectable heparin-Laponite hydrogel bridge FGF4 for spinal cord injury by stabilizing microtubule and improving mitochondrial function.Theranostics · 2019Article
- Next-generation antibodies for post-translational modifications.Current opinion in structural biology · 2018Review
- Shp2-Mitogen-Activated Protein Kinase Signaling Drives Proliferation during Zebrafish Embryo Caudal Fin Fold Regeneration.Molecular and cellular biology · 2018Article
- SHP2-Mediated Signal Networks in Stem Cell Homeostasis and Dysfunction.Stem cells international · 2018Review
- Rational Redesign of a Functional Protein Kinase-Substrate Interaction.ACS chemical biology · 2017Article
- Monobodies and other synthetic binding proteins for expanding protein science.Protein science : a publication of the Protein Society · 2017Review
- Monobodies as possible next-generation protein therapeutics - a perspective.Swiss medical weekly · 2017Article
- Antigen clasping by two antigen-binding sites of an exceptionally specific antibody for histone methylation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Conformation-Dependent Human p52Shc Phosphorylation by Human c-Src.Biochemistry · 2015Article
- Analysis on the interaction domain of VirG and apyrase by pull-down assay.Molecules (Basel, Switzerland) · 2014Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Cell signaling depends on dynamic protein-protein interaction (PPI) networks, often assembled through modular domains each interacting with multiple peptide motifs. This complexity raises a conceptual challenge, namely to define whether a particular cellular response requires assembly of the complete PPI network of interest or can be driven by a specific interaction. To address this issue, we designed variants of the Grb2 SH2 domain ("pY-clamps") whose specificity is highly biased toward a single phosphotyrosine (pY) motif among many potential pYXNX Grb2-binding sites. Surprisingly, directing Grb2 predominantly to a single pY site of the Ptpn11/Shp2 phosphatase, but not other sites tested, was sufficient for differentiation of the essential primitive endoderm lineage from embryonic stem cells. Our data suggest that discrete connections within complex PPI networks can underpin regulation of particular biological events. We propose that this directed wiring approach will be of general utility in functionally annotating specific PPIs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.