Evidence map›Paper›PMID 24909865›Full record

ArticleNuclear medicine and biology2014

Simultaneous ECG-gated PET imaging of multiple mice.

Jurgen Seidel, Marcelino L Bernardo, Karen J Wong, Biying Xu, Mark R Williams, Frank Kuo, Elaine M Jagoda, Falguni Basuli, Changhui Li, Gary L Griffiths and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nuclear medicine and biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jurgen SeidelMolecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Contractor to Leidos Biomedical Research, Inc., Frederick, MD, USA. Electronic address: jseidel1@mail.nih.gov.
Marcelino L BernardoMolecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Leidos Biomedical Research, Inc., Frederick, MD, USA.
Karen J WongMolecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Biying XuImaging Probe Development Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA; Kelly Services Inc., Troy, MI, USA.
Mark R WilliamsMolecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Leidos Biomedical Research, Inc., Frederick, MD, USA.
Frank KuoMolecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Elaine M JagodaMolecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Falguni BasuliImaging Probe Development Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA; Kelly Services Inc., Troy, MI, USA.
Changhui LiImaging Probe Development Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA; Kelly Services Inc., Troy, MI, USA.
Gary L GriffithsMolecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Clinical Research Directorate/CMRP, Leidos Biomedical Research, Inc. (formerly SAIC-Frederick, Inc.) Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Michael V GreenMolecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Contractor to Leidos Biomedical Research, Inc., Frederick, MD, USA.
Peter L ChoykeMolecular Imaging Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

Funding

Imaging Probe Development CenterZICHL005090 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI SWENSON, ROLF · 2011 to 2025
$22.2M
Instrumentation for microSPECT and microPET imagingZIABC010668 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI CHOYKE, PETER L · 2009 to 2025
$11.0M
CCR NIH HHS HHSN261200800001CIntramural NIH HHS ZIA BC010668NCI NIH HHS HHSN261200800001E
6 · The paper itself

Abstract

introductionWe describe and illustrate a method for creating ECG-gated PET images of the heart for each of several mice imaged at the same time. The method is intended to increase "throughput" in PET research studies of cardiac dynamics or to obtain information derived from such studies, e.g. tracer concentration in end-diastolic left ventricular blood.

methodsAn imaging bed with provisions for warming, anesthetic delivery, etc., was fabricated by 3D printing to allow simultaneous PET imaging of two side-by-side mice. After electrode attachment, tracer injection and placement of the animals in the scanner field of view, ECG signals from each animal were continuously analyzed and independent trigger markers generated whenever an R-wave was detected in each signal. PET image data were acquired in "list" mode and these trigger markers were inserted into this list along with the image data. Since each mouse is in a different spatial location in the FOV, sorting of these data using trigger markers first from one animal and then the other yields two independent and correctly formed ECG-gated image sequences that reflect the dynamical properties of the heart during an "average" cardiac cycle.

resultsThe described method yields two independent ECG-gated image sequences that exhibit the expected properties in each animal, e.g. variation of the ventricular cavity volumes from maximum to minimum and back during the cardiac cycle in the processed animal with little or no variation in these volumes during the cardiac cycle in the unprocessed animal.

conclusionECG-gated image sequences for each of several animals can be created from a single list mode data collection using the described method. In principle, this method can be extended to more than two mice (or other animals) and to other forms of physiological gating, e.g. respiratory gating, when several subjects are imaged at the same time.

Indexed as

AnimalsCardiac-Gated Imaging TechniquesMicePositron-Emission TomographyTime Factors[(18)F] albuminBlood pool imagingECG-gated PET imagingPre-clinical cardiac PET imaging

Identifiers

PMID24909865
PMCPMC4104247

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.