ArticleBMC genomics2014
The transcriptome of lae1 mutants of Trichoderma reesei cultivated at constant growth rates reveals new targets of LAE1 function.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- The Impact of DNA Methylation inMicroorganisms · 2025Article
- Different Putative Methyltransferases Have Different Effects on the Expression Patterns of Cellulolytic Genes.Journal of fungi (Basel, Switzerland) · 2023Article
- AfLaeA, a Global Regulator of Mycelial Growth, Chlamydospore Production, Pathogenicity, Secondary Metabolism, and Energy Metabolism in the Nematode-Trapping FungusMicrobiology spectrum · 2023Article
- Integrated Transcriptome and Untargeted Metabolomic Analyses Revealed the Role of Methyltransferase Lae1 in the Regulation of Phospholipid Metabolism inJournal of fungi (Basel, Switzerland) · 2023Article
- Bioactive Secondary Metabolites fromMicroorganisms · 2020Review
- The Putative MethyltransferaseFrontiers in microbiology · 2020Article
- Selenate sensitivity of a laeA mutant is restored by overexpression of the bZIP protein MetR in Aspergillus fumigatus.Fungal genetics and biology : FG & B · 2018Article
- Carbon Catabolite Repression in Filamentous Fungi.International journal of molecular sciences · 2017Review
- cpsA regulates mycotoxin production, morphogenesis and cell wall biosynthesis in the fungus Aspergillus nidulans.Molecular microbiology · 2017Article
- The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans.Scientific reports · 2017Article
- A novel transcription factor specifically regulates GH11 xylanase genes inBiotechnology for biofuels · 2017Article
- Regulators of plant biomass degradation in ascomycetous fungi.Biotechnology for biofuels · 2017Review
- Recent Progress on Systems and Synthetic Biology Approaches to Engineer Fungi As Microbial Cell Factories.Current genomics · 2016Article
- The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species.Microbiology and molecular biology reviews : MMBR · 2016Review
- The Different Roles ofFrontiers in microbiology · 2016Article
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9 authors.
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Abstract
backgroundThe putative methyltransferase LaeA is a global regulator that affects the expression of multiple secondary metabolite gene clusters in several fungi. In Trichoderma reesei, its ortholog LAE1 appears to predominantly regulate genes involved in increasing competitive fitness in its environment, including expression of cellulases and polysaccharide hydrolases. A drawback in all studies related to LaeA/LAE1 function so far, however, is that the respective loss-of-function and overexpressing mutants display different growth rates. Thus some of the properties attributed to LaeA/LAE1 could be simply due to changes of the growth rate.
resultsWe cultivated T. reesei, a Δlae1 mutant and a lae1-overexpressing strain in chemostats on glucose at two different growth rates (0.075 and 0.020 h-1) which resemble growth rates at repressing and derepressing conditions, respectively. Under these conditions, the effect of modulating LAE1 expression was mainly visible in the Δlae1 mutant, whereas the overexpressing strain showed little differences to the parent strain. The effect on the expression of some gene categories identified earlier (polyketide synthases, heterokaryon incompatibility proteins, PTH11-receptors) was confirmed, but in addition GCN5-N-acetyltransferases, amino acid permeases and flavin monooxygenases were identified as so far unknown major targets of LAE1 action. LAE1 was also shown to interfere with the regulation of expression of several genes by the growth rate. About a tenth of the genes differentially expressed in the Δlae1 mutant under either growth condition were found to be clustered in the genome, but no specific gene group was associated with this phenomenon.
conclusionsOur data show that - using T. reesei LAE1 as a model - the investigation of transcriptome in regulatory mutants at constant growth rates leads to new insights into the physiological roles of the respective regulator.
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