ArticlePlant biotechnology journal2014
Enhancement of extraplastidic oil synthesis in Chlamydomonas reinhardtii using a type-2 diacylglycerol acyltransferase with a phosphorus starvation-inducible promoter.
Article in Plant biotechnology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 182 citations in OpenAlex.
- Identification and functional analysis of class 3 lipases associated with triacylglycerol metabolism in Nannochloropsis oceanica (Eustigmatophyceae).Journal of phycology · 2026Article
- Triacylglycerol synthesis in microalgae under abiotic stress: an updated integrative review.Plant & cell physiology · 2026Review
- Triacylglycerols at the crossroads of lipid remodeling and stress tolerance in plants.Plant cell reports · 2026Review
- Target of Rapamycin Coordinates Metabolic Remodeling at the Protein Level in the Red AlgaPlants (Basel, Switzerland) · 2026Article
- Phosphorus Acquisition Strategies Among Phytoplankton and Free-Living Bacterial Communities in the Baltic Proper.Environmental microbiology reports · 2026Article
- Expression of the peanut diacylglycerol acyltransferase 3 increases the neutral lipid content and improves the fatty acid composition ofFrontiers in plant science · 2026Article
- Metabolic Engineering of Microalgae for Biofuel Production.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Harnessing microalgae for bioproducts: innovations in synthetic biology.World journal of microbiology & biotechnology · 2025Review
- Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria.FEBS open bio · 2025Article
- Solving Challenges in Microalgae-Based Living Materials.ACS synthetic biology · 2025Article
- Imaging Plant Lipids with Fluorescent Reporters.Plants (Basel, Switzerland) · 2024Review
- Low-dose ionizing radiation generates a hormetic response to modify lipid metabolism in Chlorella sorokiniana.Communications biology · 2024Article
- A Blue Light-Responsive Strong Synthetic Promoter Based on Rational Design inInternational journal of molecular sciences · 2023Article
- Comparison of the effects of nitrogen-, sulfur- and combined nitrogen- and sulfur-deprivations on cell growth, lipid bodies and gene expressions in Chlamydomonas reinhardtii cc5373-sta6.BMC biotechnology · 2023Article
- Novel context-specific genome-scale modelling explores the potential of triacylglycerol production by Chlamydomonas reinhardtii.Microbial cell factories · 2023Article
- Increased Lipids inInternational journal of molecular sciences · 2022Article
- Genetic compensation of triacylglycerol biosynthesis in the green microalga Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2022Article
- Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production.Marine drugs · 2022Review
- Enhanced accumulation of oil through co-expression of fatty acid and ABC transporters in Chlamydomonas under standard growth conditions.Biotechnology for biofuels and bioproducts · 2022Article
- Macular pigment-enriched oil production from genome-edited microalgae.Microbial cell factories · 2022Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When cultivated under stress conditions, many plants and algae accumulate oil. The unicellular green microalga Chlamydomonas reinhardtii accumulates neutral lipids (triacylglycerols; TAGs) during nutrient stress conditions. Temporal changes in TAG levels in nitrogen (N)- and phosphorus (P)-starved cells were examined to compare the effects of nutrient depletion on TAG accumulation in C. reinhardtii. TAG accumulation and fatty acid composition were substantially changed depending on the cultivation stage before nutrient starvation. Profiles of TAG accumulation also differed between N and P starvation. Logarithmic-growth-phase cells diluted into fresh medium showed substantial TAG accumulation with both N and P deprivation. N deprivation induced formation of oil droplets concomitant with the breakdown of thylakoid membranes. In contrast, P deprivation substantially induced accumulation of oil droplets in the cytosol and maintaining thylakoid membranes. As a consequence, P limitation accumulated more TAG both per cell and per culture medium under these conditions. To enhance oil accumulation under P deprivation, we constructed a P deprivation-dependent overexpressor of a Chlamydomonas type-2 diacylglycerol acyl-CoA acyltransferase (DGTT4) using a sulphoquinovosyldiacylglycerol 2 (SQD2) promoter, which was up-regulated during P starvation. The transformant strongly enhanced TAG accumulation with a slight increase in 18 : 1 content, which is a preferred substrate of DGTT4. These results demonstrated enhanced TAG accumulation using a P starvation-inducible promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.