Evidence map›Paper›PMID 24908390›Full record

ArticleNature immunology2014

Quantitative and temporal requirements revealed for Zap70 catalytic activity during T cell development.

Byron B Au-Yeung, Heather J Melichar, Jenny O Ross, Debra A Cheng, Julie Zikherman, Kevan M Shokat, Ellen A Robey, Arthur Weiss

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature immunology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 79 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. T-cell virtuosity in ''knowing thyself".Frontiers in immunology · 2024
    Review
  9. Article
  10. Article
  11. A Beginner's Guide to T Cell Development.Methods in molecular biology (Clifton, N.J.) · 2023
    Review
  12. Article
  13. Review
  14. The ICOS-ICOSL pathway tunes thymic selection.Immunology and cell biology · 2022
    Article
  15. Article
  16. Review
  17. T cells selectively filter oscillatory signals on the minutes timescale.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  18. Article
  19. Article
  20. Exhausted CD8Nature communications · 2020
    Article
4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Byron B Au-Yeung1] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA. [2] Rosalind Russell-Ephraim P. Engleman Rheumatology Research Center, University of California, San Francisco, San Francisco, California, USA. [3] Department of Medicine, University of California, San Francisco, San Francisco, California, USA. [4] Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA. [5].ORCID 0000000264469102
Heather J Melichar1] Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, USA. [2] [3].
Jenny O RossDivision of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, USA.
Debra A Cheng1] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA. [2] Rosalind Russell-Ephraim P. Engleman Rheumatology Research Center, University of California, San Francisco, San Francisco, California, USA. [3] Department of Medicine, University of California, San Francisco, San Francisco, California, USA. [4] Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.
Julie Zikherman1] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA. [2] Rosalind Russell-Ephraim P. Engleman Rheumatology Research Center, University of California, San Francisco, San Francisco, California, USA. [3] Department of Medicine, University of California, San Francisco, San Francisco, California, USA. [4] Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.
Kevan M Shokat1] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA. [2] Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, California, USA.
Ellen A RobeyDivision of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, USA.
Arthur Weiss1] Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA. [2] Rosalind Russell-Ephraim P. Engleman Rheumatology Research Center, University of California, San Francisco, San Francisco, California, USA. [3] Department of Medicine, University of California, San Francisco, San Francisco, California, USA. [4] Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA.
Howard Hughes Medical Institute · USUniversity of California, Berkeley · USUniversity of California, San Francisco · US

Funding

Understand the metabolic fitness of naïve T cellsP01AI091580 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ARTHUR Robert SALOMON · 2011 to 2026
$31.1M
Signals That Control Thymocyte MigrationR01AI064227 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI ELLEN A ROBEY · 2006 to 2026
$7.4M
An allosteric inhibitor of ZAP-70 as a novel therapeutic for autoimmune diseaseRC2AR058947 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WEISS, ARTHUR · 2009 to 2010
$1.8M
Titrating CD45 expression in mice to study development of T cell toleranceK08AR059723 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ZIKHERMAN, JULIE · 2010 to 2014
$598k
Howard Hughes Medical InstituteNIAID NIH HHS AI064227NIAID NIH HHS AI091580NIAID NIH HHS P01 AI091580NIAID NIH HHS R01 AI064227NIAMS NIH HHS K08 AR059723NIAMS NIH HHS RC2 AR058947NIAMS NIH HHS RC2AR058947
6 · The paper itself

Abstract

The catalytic activity of Zap70 is crucial for T cell antigen receptor (TCR) signaling, but the quantitative and temporal requirements for its function in thymocyte development are not known. Using a chemical-genetic system to selectively and reversibly inhibit Zap70 catalytic activity in a model of synchronized thymic selection, we showed that CD4(+)CD8(+) thymocytes integrate multiple, transient, Zap70-dependent signals over more than 36 h to reach a cumulative threshold for positive selection, whereas 1 h of signaling was sufficient for negative selection. Titration of Zap70 activity resulted in graded reductions in positive and negative selection but did not decrease the cumulative TCR signals integrated by positively selected OT-I cells, which revealed heterogeneity, even among CD4(+)CD8(+) thymocytes expressing identical TCRs undergoing positive selection.

Indexed as

AnimalsCalciumCatalysisCell DifferentiationIntracellular Signaling Peptides and ProteinsMiceMice, Inbred C57BLProtein-Tyrosine KinasesReceptors, Antigen, T-CellSignal TransductionSyk KinaseT-LymphocytesZAP-70 Protein-Tyrosine KinaseCalciumIntracellular Signaling Peptides and ProteinsProtein-Tyrosine KinasesReceptors, Antigen, T-CellSyk KinaseSyk protein, mouseZap70 protein, mouseZAP-70 Protein-Tyrosine Kinase

Identifiers

PMID24908390
PMCPMC4095875
OpenAlexW1982161183

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.