Evidence map›Paper›PMID 24908100›Full record

ArticleNature methods2014

Targeting cells with single vectors using multiple-feature Boolean logic.

Lief E Fenno, Joanna Mattis, Charu Ramakrishnan, Minsuk Hyun, Soo Yeun Lee, Miao He, Jason Tucciarone, Aslihan Selimbeyoglu, Andre Berndt, Logan Grosenick and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 374 papers.

0numbers the graph read from it
0cells of the map it votes in
374citing papers in PubMed
17.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

374 citing papers in PubMed, 571 citations in OpenAlex.

  1. Article
  2. ICER-Controlled inhibitory plasticity of amygdala engram neurons regulates fear memory precision.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026
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  4. Calbindin stratifies midbrain dopaminergic neurons governing distinct aspects of locomotion.Proceedings of the National Academy of Sciences of the United States of America · 2026
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  10. Intersectional expression ofAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2026
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314 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 6 institutions in 1 country.

Lief E Fenno1] Department of Neuroscience, Stanford University, Stanford, California, USA. [2] Department of Bioengineering, Stanford University, Stanford, California, USA. [3].
Joanna Mattis1] Department of Neuroscience, Stanford University, Stanford, California, USA. [2] Department of Bioengineering, Stanford University, Stanford, California, USA. [3].
Charu Ramakrishnan1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2].
Minsuk HyunDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Soo Yeun Lee1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] CNC Program, Stanford University, Stanford, California, USA.
Miao HeCold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
Jason Tucciarone1] Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA. [2] Program in Neuroscience, Stony Brook University, Stony Brook, New York, USA.
Aslihan SelimbeyogluDepartment of Neuroscience, Stanford University, Stanford, California, USA.
Andre BerndtDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Logan Grosenick1] Department of Neuroscience, Stanford University, Stanford, California, USA. [2] Department of Bioengineering, Stanford University, Stanford, California, USA. [3] CNC Program, Stanford University, Stanford, California, USA.
Kelly A Zalocusky1] Department of Neuroscience, Stanford University, Stanford, California, USA. [2] CNC Program, Stanford University, Stanford, California, USA.
Hannah BernsteinDepartment of Neuroscience and Physiology, New York University School of Medicine, New York, New York, USA.
Haley SwansonCNC Program, Stanford University, Stanford, California, USA.
Chelsey PerryCNC Program, Stanford University, Stanford, California, USA.
Ilka Diester1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] Ernst Struengmann Institute for Neuroscience, Frankfurt, Germany.
Frederick M BoyceDepartment of Neurology, Massachusetts General Hospital, Cambridge, Massachusetts, USA.
Caroline E BassDepartment of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York, USA.
Rachael NeveDepartment of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Z Josh HuangCold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
Karl Deisseroth1] Department of Bioengineering, Stanford University, Stanford, California, USA. [2] CNC Program, Stanford University, Stanford, California, USA. [3] Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, California, USA. [4] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.
Stanford University · USCold Spring Harbor Laboratory · USMassachusetts General Hospital · USMassachusetts Institute of Technology · USNew York University · USUniversity at Buffalo, State University of New York · US

Funding

CLARITY: fully-assembled biologyR01MH099647 · NIMH · STANFORD UNIVERSITY · PI DEISSEROTH, KARL A. · 2012 to 2016
$23.0M
Training Program in Basic NeuroscienceT32MH020016 · NIMH · STANFORD UNIVERSITY · PI Justin L Gardner, Merritt C Maduke · 1997 to 2026
$16.2M
OPTICAL NEUROMODULATION TECHNOLOGY FOR LONG TIMESCALESR01MH086373 · NIMH · STANFORD UNIVERSITY · PI DEISSEROTH, KARL A. · 2010 to 2025
$9.1M
VECTOR COREP30NS069375 · NINDS · STANFORD UNIVERSITY · PI OLSON, ANDREW · 2011 to 2019
$6.3M
Structural and molecular identification of circuitry underlying joint processing of motivation and aversionR37DA035377 · NIDA · STANFORD UNIVERSITY · PI DEISSEROTH, KARL A. · 2013 to 2022
$6.2M
Genetic analysis of chandelier cells during cortical circuit assemblyR01MH094705 · NIMH · DUKE UNIVERSITY · PI HUANG, Z JOSH · 2011 to 2022
$5.8M
Calcium channels, newborn neurons, and CNS circuit dynamicsR01DA020794 · NIDA · STANFORD UNIVERSITY · PI DEISSEROTH, KARL A. · 2005 to 2014
$4.1M
Technology development for fast optical interrogation of living neural circuitryR01MH075957 · NIMH · STANFORD UNIVERSITY · PI DEISSEROTH, KARL A. · 2008 to 2012
$2.6M
CB1 and DA Receptor Deletion by RNAi in Cocaine Self-AdministrationK01DA024763 · NIDA · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI BASS, CAROLINE EVELYN · 2008 to 2011
$568k
Howard Hughes Medical InstituteNIDA NIH HHS DA024763NIDA NIH HHS K01 DA024763NIDA NIH HHS R01 DA020794NIDA NIH HHS R37 DA035377NIMH NIH HHS R01 MH075957NIMH NIH HHS R01 MH086373NIMH NIH HHS R01 MH094705NIMH NIH HHS R01 MH099647NIMH NIH HHS T32 MH020016NINDS NIH HHS P30 NS069375NINDS NIH HHS P30 NS069375-01A1
6 · The paper itself

Abstract

Precisely defining the roles of specific cell types is an intriguing frontier in the study of intact biological systems and has stimulated the rapid development of genetically encoded tools for observation and control. However, targeting these tools with adequate specificity remains challenging: most cell types are best defined by the intersection of two or more features such as active promoter elements, location and connectivity. Here we have combined engineered introns with specific recombinases to achieve expression of genetically encoded tools that is conditional upon multiple cell-type features, using Boolean logical operations all governed by a single versatile vector. We used this approach to target intersectionally specified populations of inhibitory interneurons in mammalian hippocampus and neurons of the ventral tegmental area defined by both genetic and wiring properties. This flexible and modular approach may expand the application of genetically encoded interventional and observational tools for intact-systems biology.

Indexed as

Genetic VectorsAnimalsBacterial ProteinsDependovirusFemaleGene TargetingHEK293 CellsHippocampusHumansIntegrasesInterneuronsIntronsLogicLuminescent ProteinsMaleMiceBacterial ProteinsCre recombinaseIntegrasesLuminescent Proteinsyellow fluorescent protein, Bacteria

Identifiers

PMID24908100
PMCPMC4085277
OpenAlexW2165147454

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.