ArticleProtist2014
Nanochromosome copy number does not correlate with RNA levels though patterns are conserved between strains of the ciliate morphospecies Chilodonella uncinata.
Article in Protist, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Genome content reorganization in the non-model ciliatemSphere · 2025Article
- Genome content in the non-model ciliatebioRxiv : the preprint server for biology · 2024Article
- Somatic genome architecture and molecular evolution are decoupled in "young" linage-specific gene families in ciliates.PloS one · 2024Article
- Transcriptomic Differences between Free-Living and ParasiticMicroorganisms · 2022Article
- Comparative Genome Analysis RevealsFrontiers in microbiology · 2022Article
- DAPI staining and DNA content estimation of nuclei in uncultivable microbial eukaryotes (Arcellinida and Ciliates).European journal of protistology · 2021Article
- Evolutionary origins and impacts of genome architecture in ciliates.Annals of the New York Academy of Sciences · 2019Review
- Twisted Tales: Insights into Genome Diversity of Ciliates Using Single-Cell 'Omics.Genome biology and evolution · 2018Article
- Article
- Nuclear Features of the Heterotrich Ciliate Blepharisma americanum: Genomic Amplification, Life Cycle, and Nuclear Inclusion.The Journal of eukaryotic microbiology · 2018Article
- Insights into transgenerational epigenetics from studies of ciliates.European journal of protistology · 2017Review
- Disentangling sources of variation in SSU rDNA sequences from single cell analyses of ciliates: impact of copy number variation and experimental error.Proceedings. Biological sciences · 2017Article
- Nuclear Architecture and Patterns of Molecular Evolution Are Correlated in the Ciliate Chilodonella uncinata.Genome biology and evolution · 2016Article
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Authors and funding
2 authors.
Funding
Abstract
In some ciliates, extensive genome fragmentation leads to a macronucleus (i.e. somatic nucleus) containing gene-sized chromosomes that vary in copy number. Yet the relationship between copy number and expression level is not well understood as previous work has shown a variety of patterns. For example, nanochromosome copy numbers are positively correlated to mRNA levels in spirotrichous ciliates, while one study of Chilodonella uncinata suggested that they were inversely correlated. To study further copy number and expression levels in C. uncinata, we analyzed 11 members in five gene families (SSU-rDNA, actin, alpha-tubulin, histidine acid phosphatase family protein and a protein kinase domain containing protein) from one strain. We find that macronuclear copy numbers of these genes range from hundreds to thousands per cell, and that copy number does not correlate with expression level as measured by steady-state RNA in predominantly-vegetative cultures. We also compared six of these genes to their orthologs in a second genetically-isolated strain of C. uncinata to reveal that patterns of nanochromosome and transcript copy numbers are conserved between strains. Our data suggest that nanochromosome copy number may be related to a feature like nuclear architecture.
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