ArticleApplied and environmental microbiology2014
Profile of secreted hydrolases, associated proteins, and SlpA in Thermoanaerobacterium saccharolyticum during the degradation of hemicellulose.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 29 citations in OpenAlex.
- Genome-Scale Metabolic Model ofmSystems · 2021Article
- Thermophilic microbial deconstruction and conversion of natural and transgenic lignocellulose.Environmental microbiology reports · 2021Review
- Metabolic Fluxes of Nitrogen and Pyrophosphate in Chemostat Cultures of Clostridium thermocellum and Thermoanaerobacterium saccharolyticum.Applied and environmental microbiology · 2020Article
- Conversion of phosphoenolpyruvate to pyruvate inMetabolic engineering communications · 2020Article
- Article
- Microbial diversity of thermophiles with biomass deconstruction potential in a foliage-rich hot spring.MicrobiologyOpen · 2018Article
- Biogeography and taxonomic overview of terrestrial hot spring thermophilic phages.Extremophiles : life under extreme conditions · 2018Review
- The redox-sensing protein Rex modulates ethanol production in Thermoanaerobacterium saccharolyticum.PloS one · 2018Article
- Lactobacillus slpA promotes ESC growth through the ERK1/2 pathway.Cytotechnology · 2017Article
- Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood.Biotechnology for biofuels · 2016Article
- Deletion of nfnAB in Thermoanaerobacterium saccharolyticum and Its Effect on Metabolism.Journal of bacteriology · 2015Article
- Cofactor Specificity of the Bifunctional Alcohol and Aldehyde Dehydrogenase (AdhE) in Wild-Type and Mutant Clostridium thermocellum and Thermoanaerobacterium saccharolyticum.Journal of bacteriology · 2015Article
- Genome-scale resources for Thermoanaerobacterium saccharolyticum.BMC systems biology · 2015Article
- The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum.Journal of bacteriology · 2015Article
- Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485.Biotechnology for biofuels · 2015Article
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9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thermoanaerobacterium saccharolyticum, a Gram-positive thermophilic anaerobic bacterium, grows robustly on insoluble hemicellulose, which requires a specialized suite of secreted and transmembrane proteins. We report here the characterization of proteins secreted by this organism. Cultures were grown on hemicellulose, glucose, xylose, starch, and xylan in pH-controlled bioreactors, and samples were analyzed via spotted microarrays and liquid chromatography-mass spectrometry. Key hydrolases and transporters employed by T. saccharolyticum for growth on hemicellulose were, for the most part, hitherto uncharacterized and existed in two clusters (Tsac_1445 through Tsac_1464 for xylan/xylose and Tsac_1344 through Tsac_1349 for starch). A phosphotransferase system subunit, Tsac_0032, also appeared to be exclusive to growth on glucose. Previously identified hydrolases that showed strong conditional expression changes included XynA (Tsac_1459), XynC (Tsac_0897), and a pullulanase, Apu (Tsac_1342). An omnipresent transcript and protein making up a large percentage of the overall secretome, Tsac_0361, was tentatively identified as the primary S-layer component in T. saccharolyticum, and deletion of the Tsac_0361 gene resulted in gross morphological changes to the cells. The view of hemicellulose degradation revealed here will be enabling for metabolic engineering efforts in biofuel-producing organisms that degrade cellulose well but lack the ability to catabolize C5 sugars.
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