Evidence map›Paper›PMID 24907332›Full record

ArticleApplied and environmental microbiology2014

Identification and characterization of a halotolerant, cold-active marine endo-β-1,4-glucanase by using functional metagenomics of seaweed-associated microbiota.

Marjolaine Martin, Sophie Biver, Sébastien Steels, Tristan Barbeyron, Murielle Jam, Daniel Portetelle, Gurvan Michel, Micheline Vandenbol

Open access · bronzeAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
9.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 54 citations in OpenAlex.

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  10. Recent Advances in Function-based Metagenomic Screening.Genomics, proteomics & bioinformatics · 2018
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Marjolaine MartinMicrobiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium marjolaine.martin@ulg.ac.be.
Sophie BiverMicrobiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium.
Sébastien SteelsMicrobiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium.
Tristan BarbeyronSorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France.
Murielle JamSorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France.
Daniel PortetelleMicrobiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium.
Gurvan MichelSorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff, Bretagne, France.
Micheline VandenbolMicrobiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium.
University of Liège · BECentre National de la Recherche Scientifique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions.

Indexed as

MicrobiotaBacteriaBacterial ProteinsCold TemperatureEnzyme StabilityGlycoside HydrolasesMetagenomicsMolecular Sequence DataPhaeophyceaePhylogenySeaweedSodium ChlorideBacterial ProteinsGlycoside HydrolasesSodium Chloride

Identifiers

PMID24907332
PMCPMC4135742
OpenAlexW2129726221

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.