ArticleApplied and environmental microbiology2014
Revision of N2O-producing pathways in the ammonia-oxidizing bacterium Nitrosomonas europaea ATCC 19718.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 151 citations in OpenAlex.
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- The microbial nexus: linking arsenic biogeochemistry with greenhouse gas emissions.Frontiers in microbiology · 2026Review
- Continuous N supply at a low temperature produces less NFrontiers in microbiology · 2026Article
- Oxygen production via nitric oxide dismutation in diverse ammonia oxidizers.ISME communications · 2026Article
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- Dating Ammonia-Oxidizing Bacteria with Abundant Eukaryotic Fossils.Molecular biology and evolution · 2024Article
- Role of Nitric Oxide in Hydroxylamine Oxidation by Ammonia-Oxidizing Bacteria.Applied and environmental microbiology · 2023Article
- Proterozoic Acquisition of Archaeal Genes for Extracellular Electron Transfer: A Metabolic Adaptation of Aerobic Ammonia-Oxidizing Bacteria to Oxygen Limitation.Molecular biology and evolution · 2023Article
- Effects of acidification on nitrification and associated nitrous oxide emission in estuarine and coastal waters.Nature communications · 2023Article
- Temperature dependence of nitrification in a membrane-aerated biofilm reactor.Frontiers in microbiology · 2023Article
- NFrontiers in bioengineering and biotechnology · 2023Review
- The contributions of ammonia oxidizing bacteria and archaea to nitrification-dependent NScientific reports · 2022Article
- Black Truffles AffectFrontiers in microbiology · 2022Article
- Ammonia-oxidizing archaea in biological interactions.Journal of microbiology (Seoul, Korea) · 2021Review
- Transcriptomic Response of Nitrosomonas europaea Transitioned from Ammonia- to Oxygen-Limited Steady-State Growth.mSystems · 2020Article
- A Physiological and Genomic Comparison of Nitrosomonas Cluster 6a and 7 Ammonia-Oxidizing Bacteria.Microbial ecology · 2019Article
- Low yield and abiotic origin of NNature communications · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrite reductase (NirK) and nitric oxide reductase (NorB) have long been thought to play an essential role in nitrous oxide (N2O) production by ammonia-oxidizing bacteria. However, essential gaps remain in our understanding of how and when NirK and NorB are active and functional, putting into question their precise roles in N2O production by ammonia oxidizers. The growth phenotypes of the Nitrosomonas europaea ATCC 19718 wild-type and mutant strains deficient in expression of NirK, NorB, and both gene products were compared under atmospheric and reduced O2 tensions. Anoxic resting-cell assays and instantaneous nitrite (NO2 (-)) reduction experiments were done to assess the ability of the wild-type and mutant N. europaea strains to produce N2O through the nitrifier denitrification pathway. Results confirmed the role of NirK for efficient substrate oxidation of N. europaea and showed that NorB is involved in N2O production during growth at both atmospheric and reduced O2 tensions. Anoxic resting-cell assays and measurements of instantaneous NO2 (-) reduction using hydrazine as an electron donor revealed that an alternate nitrite reductase to NirK is present and active. These experiments also clearly demonstrated that NorB was the sole nitric oxide reductase for nitrifier denitrification. The results of this study expand the enzymology for nitrogen metabolism and N2O production by N. europaea and will be useful to interpret pathways in other ammonia oxidizers that lack NirK and/or NorB genes.
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