ArticlePhysiological reports2014
Chylomicrons produced by Caco-2 cells contained ApoB-48 with diameter of 80-200 nm.
Article in Physiological reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Primary Small Intestinal Epithelia Are Physiological Alternatives to the Caco-2 Lymphatic Transport Model.Molecular pharmaceutics · 2025Article
- Review
- Next-Generation Lipid Prodrugs Orally Deliver Tenofovir via Enhanced Chylomicron Incorporation.ACS pharmacology & translational science · 2025Article
- Separation of Lipoproteins for Quantitative Analysis ofInternational journal of molecular sciences · 2024Article
- Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification.Beilstein journal of nanotechnology · 2024Article
- A Shared Perspective onJournal of agricultural and food chemistry · 2023Review
- Validation of Knock-Out Caco-2 TC7 Cells as Models of Enterocytes of Patients with Familial Genetic Hypobetalipoproteinemias.Nutrients · 2023Article
- The effects of sex hormones on the size of intestinal lipoproteins.Frontiers in physiology · 2023Article
- Food matrix impacts bioaccessibility and assimilation of acid whey-derived milk fat globule membrane lipids in Caco-2 cells.Frontiers in nutrition · 2023Article
- Dose Dependent Effects of Fructose and Glucose on de novo Palmitate and Glycerol Synthesis in an Enterocyte Cell Model.Molecular nutrition & food research · 2022Article
- Article
- Article
- Why Do Men Accumulate Abdominal Visceral Fat?Frontiers in physiology · 2019Article
- Influence of PEGylation of Vitamin-K-Loaded Mixed Micelles on the Uptake by and Transport through Caco-2 Cells.Molecular pharmaceutics · 2018Article
- Key differences between apoC-III regulation and expression in intestine and liver.Biochemical and biophysical research communications · 2017Article
- Using primary murine intestinal enteroids to study dietary TAG absorption, lipoprotein synthesis, and the role of apoC-III in the intestine.Journal of lipid research · 2017Article
- Recent discoveries on absorption of dietary fat: Presence, synthesis, and metabolism of cytoplasmic lipid droplets within enterocytes.Biochimica et biophysica acta · 2016Review
- TANGO1 and Mia2/cTAGE5 (TALI) cooperate to export bulky pre-chylomicrons/VLDLs from the endoplasmic reticulum.The Journal of cell biology · 2016Article
- Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection.Scientific reports · 2016Article
- Using Caco-2 Cells to Study Lipid Transport by the Intestine.Journal of visualized experiments : JoVE · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The small intestine generally transports dietary fats to circulation in triglyceride (TG)-rich lipoproteins. The two main intestinal lipoproteins are chylomicron (CM) and very low-density lipoprotein (VLDL). Unfortunately, studies on the CM biogenesis and intestinal transport of dietary fats have been hampered by the lack of an adequate in vitro model. In this study, we investigated the possible factors that might increase the efficiency of CM production by Caco-2 cells. We utilized sequential NaCl gradient ultracentrifugation to isolate the CMs that were secreted by the Caco-2 cells. To confirm the successful isolation of the CMs, we performed Fat Red 7B staining, TG reading, apolipoprotein B (ApoB) measurement, and transmission electron microcopy (TEM) analysis. We then tested the effects of cell differentiation, oleic acid, mono-olein, egg lecithin, incubation time, and collagen matrix on CM secretion. We found that cell differentiation, oleic acid, and lecithin were critical for CM secretion. Using the Transwell system, we further confirmed that the CMs produced by our Caco-2 cells contained significant amount of TGs and ApoB-48 such that they could be detected without the use of isotope labeling. In conclusion, when fully differentiated Caco-2 were challenged with oleic acid, lecithin, and sodium taurocholate, 21% of their total number of lipoproteins were CMs with the diameter of 80-200 nm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.