ArticleParasitology research2014
Trypanosoma cruzi-secreted vesicles have acid and alkaline phosphatase activities capable of increasing parasite adhesion and infection.
Article in Parasitology research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
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- Towards a map of the immune system manipulation network byFrontiers in cellular and infection microbiology · 2025Review
- RNA-containing extracellular vesicles in infection.RNA biology · 2024Review
- Biogenesis of extracellular vesicles in protozoan parasites: The ESCRT complex in the trafficking fast lane?PLoS pathogens · 2023Review
- Extracellular Vesicles inJournal of immunology research · 2022Review
- An induced population ofFrontiers in cellular and infection microbiology · 2022Article
- Extracellular vesicles fromFrontiers in cellular and infection microbiology · 2022Article
- In Silico Characterization of Calcineurin from Pathogenic Obligate Intracellular Trypanosomatids: Potential New Biological Roles.Biomolecules · 2021Article
- AgNP-PVP-meglumine antimoniate nanocomposite reduces Leishmania amazonensis infection in macrophages.BMC microbiology · 2021Article
- The Road Less Traveled? Unconventional Protein Secretion at Parasite-Host Interfaces.Frontiers in cell and developmental biology · 2021Review
- Impact of the Extracellular Vesicles Derived FromFrontiers in cellular and infection microbiology · 2021Review
- Extracellular Vesicles: Potential Role in Remote Signaling and Inflammation inFrontiers in cell and developmental biology · 2021Review
- Quantitative proteomic analysis and functional characterization of Acanthamoeba castellanii exosome-like vesicles.Parasites & vectors · 2019Article
- Measurement of Acid Ecto-phosphatase Activity in LiveBio-protocol · 2019Article
- Message in a vesicle - trans-kingdom intercommunication at the vector-host interface.Journal of cell science · 2019Review
- Extracellular vesicles of Trypanosoma cruzi tissue-culture cell-derived trypomastigotes: Induction of physiological changes in non-parasitized culture cells.PLoS neglected tropical diseases · 2019Article
- Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation.Parasites & vectors · 2018Article
- Extracellular Vesicles Shed ByFrontiers in immunology · 2018Article
- Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells.Journal of clinical microbiology · 2017Article
- Deep Insight into the Phosphatomes of Parasitic Protozoa and a Web Resource ProtozPhosDB.PloS one · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypanosoma cruzi virulence factors include molecules expressed on the cell surface as well as those secreted or shed into the extracellular medium. Phosphatase activities modulate different aspects of T. cruzi infection, although no studies to date addressed the presence and activity of phosphatases in vesicles secreted by this parasite. Here, we characterized acidic and alkaline secreted phosphatase activities of human-infective trypomastigote forms of T. cruzi from the Y strain and the CL-Brener clone. These are widely studied T. cruzi strains that represent "opposite ends of the spectrum" regarding both in vitro and in vivo behavior. Ecto-phosphatase activities were determined in live parasites, and secreted phosphatase activities were analyzed in soluble protein (SP) and vesicular membrane fractions (VFs) of parasite-conditioned medium. Our analysis using different phosphatase inhibitors strongly suggests that vesicles secreted by Y strain (VF(Y)) and CL-Brener (VF(CLB)) trypomastigotes are derived mostly from the cell surface and from exosome secretion, respectively. Importantly, our results show that the acid phosphatase activities in vesicles secreted by trypomastigotes are largely responsible for the VF-induced increase in adhesion of Y strain parasites to host cells and also for the VF-induced increase in host cell infection by CL-Brener trypomastigotes.
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