ArticleBMC microbiology2014
The genetic diversity of cereulide biosynthesis gene cluster indicates a composite transposon Tnces in emetic Bacillus weihenstephanensis.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 39 citations in OpenAlex.
- Complete genome of a cereulide-producingMicrobiology resource announcements · 2026Article
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- Intraspecific Diversity and Pathogenicity ofToxins · 2023Article
- Preclinical Safety Assessment ofMicroorganisms · 2022Article
- Influence of the Phagemid PfNC7401 on Cereulide-ProducingMicroorganisms · 2022Article
- Conjugation acrossFrontiers in microbiology · 2022Review
- A novel anti-dipteranApplied and environmental microbiology · 2021Article
- Identification of a NovelFrontiers in microbiology · 2021Article
- Cereulide Synthetase Acquisition and Loss Events within the Evolutionary History of Group IIImBio · 2020Article
- Risk presented to minimally processed chilled foods by psychrotrophicTrends in food science & technology · 2019Review
- Diversity of Bacillus cereus sensu lato mobilome.BMC genomics · 2019Article
- Characterization of the Genome Feature and Toxic Capacity of aFrontiers in cellular and infection microbiology · 2019Article
- A Comparative Analysis of Biosynthetic Gene Clusters in Lean and Obese Humans.BioMed research international · 2019Article
- Identification and genomic comparison of temperate bacteriophages derived from emetic Bacillus cereus.PloS one · 2017Article
- Genetic interrelations in the actinomycin biosynthetic gene clusters ofAdvances and applications in bioinformatics and chemistry : AABC · 2017Article
- Food-bacteria interplay: pathometabolism of emetic Bacillus cereus.Frontiers in microbiology · 2015Review
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6 authors at 4 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCereulide is a cyclic dodecadepsipeptide ionophore, produced via non-ribosomal peptide synthetases (NRPS), which in rare cases can lead to human death. Early studies had shown that emetic toxin formation belongs to a homogeneous group of Bacillus cereus sensu stricto and the genetic determinants of cereulide (a 24-kb gene cluster of cesHPTABCD) are located on a 270-kb plasmid related to the Bacillus anthracis virulence plasmid pXO1.
resultsThe whole genome sequences from seven emetic isolates, including two B. cereus sensu stricto and five Bacillus weihenstephanensis strains, were compared, and their inside and adjacent DNA sequences of the cereulide biosynthesis gene clusters were analyzed. The sequence diversity was observed, which classified the seven emetic isolates into three clades. Different genomic locations of the cereulide biosynthesis gene clusters, plasmid-borne and chromosome-borne, were also found. Potential mobile genetic elements (MGEs) were identified in the flanking sequences of the ces gene cluster in all three types. The most striking observation was the identification of a putative composite transposon, Tnces, consisting of two copies of ISces element (belonging to IS6 family) in opposite orientations flanking the ces gene cluster in emetic B. weihenstephanensis. The mobility of this element was tested by replacing the ces gene cluster by a KmR gene marker and performing mating-out transposition assays in Escherichia coli. The results showed that Tnces::km transposes efficiently (1.04 × 10(-3) T/R) and produces 8-bp direct repeat (DR) at the insertion sites.
conclusionsCereulide biosynthesis gene clusters display sequence diversity, different genomic locations and association with MGEs, in which the transposition capacity of a resistant derivative of the composite transposon Tnces in E. coli was demonstrated. Further study is needed to look for appropriate genetic tools to analysis the transposition of Tnces in Bacillus spp. and the dynamics of other MGEs flanking the ces gene clusters.
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