ArticleColloids and surfaces. B, Biointerfaces2014
Binding interactions of bacterial lipopolysaccharide and the cationic amphiphilic peptides polymyxin B and WLBU2.
Article in Colloids and surfaces. B, Biointerfaces, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Polymyxins slow down lateral diffusion of proteins and lipopolysaccharide in the E. coli outer membrane.Communications biology · 2025Article
- Antimicrobial peptide 2K4L inhibits the inflammatory response in macrophages and Caenorhabditis elegans and protects against LPS-induced septic shock in mice.Scientific reports · 2024Article
- Insights into colistin-mediated fluorescence labelling of bacterial LPS.RSC advances · 2024Article
- Functionalized core-shell nanogel scavenger for immune modulation therapy in sepsis.Advanced therapeutics · 2022Article
- Atomic-Resolution Structures and Mode of Action of Clinically Relevant Antimicrobial Peptides.International journal of molecular sciences · 2022Review
- The Engineered Antibiotic Peptide PLG0206 Eliminates Biofilms and Is a Potential Treatment for Periprosthetic Joint Infections.Antibiotics (Basel, Switzerland) · 2021Review
- Polymyxin B1 within theBiophysical reviews · 2021Review
- Touchable 3D hierarchically structured polyaniline nanoweb for capture and detection of pathogenic bacteria.Nano convergence · 2021Article
- Connection between Periodontitis-Induced Low-Grade Endotoxemia and Systemic Diseases: Neutrophils as Protagonists and Targets.International journal of molecular sciences · 2021Review
- Antibiofilm peptides as a promising strategy: comparative research.Applied microbiology and biotechnology · 2021Article
- Engineered Cationic Antimicrobial Peptides (eCAPs) to Combat Multidrug-Resistant Bacteria.Pharmaceutics · 2020Review
- Photocatalytically renewable peptide-based electrochemical impedance method for sensing lipopolysaccharide.Mikrochimica acta · 2020Article
- Simplified Head-to-Tail Cyclic Polypeptides as Biomaterial-Associated Antimicrobials with Endotoxin Neutralizing and Anti-Inflammatory Capabilities.International journal of molecular sciences · 2019Article
- PolyBall: A new adsorbent for the efficient removal of endotoxin from biopharmaceuticals.Scientific reports · 2019Article
- Prevention of ESKAPE pathogen biofilm formation by antimicrobial peptides WLBU2 and LL37.International journal of antimicrobial agents · 2018Article
- The Perturbation of Pulmonary Surfactant by Bacterial Lipopolysaccharide and Its Reversal by Polymyxin B: Function and Structure.International journal of molecular sciences · 2018Article
- A Rapid and Semi-Quantitative Gold Nanoparticles Based Strip Sensor for Polymyxin B Sulfate Residues.Nanomaterials (Basel, Switzerland) · 2018Article
- Antimicrobial Peptides: A Potential Therapeutic Option for Surgical Site Infections.Clinics in surgery · 2017Article
- A synthetic cationic antimicrobial peptide inhibits inflammatory response and the NLRP3 inflammasome by neutralizing LPS and ATP.PloS one · 2017Article
- Clinical potential of engineered cationic antimicrobial peptides against drug resistant biofilms.Expert review of anti-infective therapy · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Passage of blood through a sorbent device for removal of bacteria and endotoxin by specific binding with immobilized, membrane-active, bactericidal peptides holds promise for treating severe blood infections. Peptide insertion in the target membrane and rapid/strong binding is desirable, while membrane disruption and release of degradation products to the circulating blood is not. Here we describe interactions between bacterial endotoxin (lipopolysaccharide, LPS) and the membrane-active, bactericidal peptides WLBU2 and polymyxin B (PmB). Analysis of the interfacial behavior of mixtures of LPS and peptide using air-water interfacial tensiometry and optical waveguide lightmode spectroscopy strongly suggests insertion of intact LPS vesicles by the peptide WLBU2 without vesicle destabilization. In contrast, dynamic light scattering (DLS) studies show that LPS vesicles appear to undergo peptide-induced destabilization in the presence of PmB. Circular dichroism spectra further confirm that WLBU2, which shows disordered structure in aqueous solution and substantially helical structure in membrane-mimetic environments, is stably located within the LPS membrane in peptide-vesicle mixtures. We therefore expect that presentation of WLBU2 at an interface, if tethered in a fashion which preserves its mobility and solvent accessibility, will enable the capture of bacteria and endotoxin without promoting reintroduction of endotoxin to the circulating blood, thus minimizing adverse clinical outcomes. On the other hand, our results suggest no such favorable outcome of LPS interactions with polymyxin B.
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