ArticleACS synthetic biology2014
Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides.
Article in ACS synthetic biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- A molecular integrator of sleep duration and interruption.bioRxiv : the preprint server for biology · 2026Article
- A novel directed evolution platform for engineering chemically gated protein switches.Chemical communications (Cambridge, England) · 2026Article
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
- Engineering filamentous myosins for optical control of contractility.bioRxiv : the preprint server for biology · 2025Article
- Design of a light and CaProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cardiac optogenetics: shining light on signaling pathways.Pflugers Archiv : European journal of physiology · 2023Review
- Photoswitchable Inhibitors to Optically Control Specific Kinase Activity.ACS chemical biology · 2023Article
- LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics.Biophysics and physicobiology · 2023Article
- Study of spatiotemporal regulation of kinase signaling using genetically encodable molecular tools.Current opinion in chemical biology · 2022Review
- Deactivatable Bisubstrate Inhibitors of Protein Kinases.Molecules (Basel, Switzerland) · 2022Article
- Article
- Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies.Nature methods · 2022Article
- Genetically encoded sensors towards imaging cAMP and PKA activity in vivo.Journal of neuroscience methods · 2021Review
- A guide to the optogenetic regulation of endogenous molecules.Nature methods · 2021Review
- Optogenetics: The Art of Illuminating Complex Signaling Pathways.Physiology (Bethesda, Md.) · 2021Review
- Engineering Optogenetic Protein Analogs.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Protein Kinase Inhibitor Peptide as a Tool to Specifically Inhibit Protein Kinase A.Frontiers in physiology · 2020Review
- Optogenetic Techniques for Manipulating and Sensing G Protein-Coupled Receptor Signaling.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Optogenetic control of cofilin and αTAT in living cells using Z-lock.Nature chemical biology · 2019Article
- Optical control of protein phosphatase function.Nature communications · 2019Article
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Authors and funding
5 authors.
Funding
Abstract
Optogenetic control of endogenous signaling can be an important tool for probing cell behavior. Using the photoresponse of the LOV2 domain of Avena sativa phototropin 1, we developed analogues of kinase inhibitors whose activity is light dependent. Inhibitory peptides were appended to the Jα helix, where they potently inhibited kinases in the light but were sterically blocked from kinase interaction in the dark. Photoactivatable inhibitors for cyclic-AMP dependent kinase (PKA) and myosin light chain kinase (MLCK) are described, together with studies that shed light on proper positioning of the peptides in the LOV domain. These inhibitors altered endogenous signaling in living cells and produced light-dependent changes in cell morphodynamics.
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Registered trials
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