ArticleInternational journal of molecular sciences2014
A comprehensive insight into tetracycline resistant bacteria and antibiotic resistance genes in activated sludge using next-generation sequencing.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Concordance between environmental resistomes and pathogenic phenotypes: a case study of multidrug-resistantFrontiers in microbiology · 2026Article
- Modulation of gut microbiota and intestinal barrier by lotus seed, jujube, and longan aril in senna leaf-induced diarrhea in mice.Frontiers in microbiology · 2026Article
- Impact of Silver and Copper Oxide Nanoparticles on Anaerobic Digestion of Sludge and Bacterial Community Structure.Nanomaterials (Basel, Switzerland) · 2025Article
- Physiological adaptation and population dynamics of a nitrifying sludge exposed to ampicillin.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Metalloporphyrin modified defective TiORSC advances · 2023Article
- Correlations among Antibiotic Resistance Genes, Mobile Genetic Elements and Microbial Communities in Municipal Sewage Treatment Plants Revealed by High-Throughput Sequencing.International journal of environmental research and public health · 2023Article
- Soil microbial communities following 20 years of fertilization and crop rotation practices in the Czech Republic.Environmental microbiome · 2022Article
- Quantification of Airborne Resistant Organisms With Temporal and Spatial Diversity in Bangladesh: Protocol for a Cross-Sectional Study.JMIR research protocols · 2019Article
- Bacterial community structures and biodegradation kinetic of Tiamulin antibiotic degrading enriched consortia from swine wastewater.Journal of environmental health science & engineering · 2019Article
- Functional Metagenomics as a Tool for Identification of New Antibiotic Resistance Genes from Natural Environments.Microbial ecology · 2017Review
- Bacterial communities in full-scale wastewater treatment systems.World journal of microbiology & biotechnology · 2016Review
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
In order to comprehensively investigate tetracycline resistance in activated sludge of sewage treatment plants, 454 pyrosequencing and Illumina high-throughput sequencing were used to detect potential tetracycline resistant bacteria (TRB) and antibiotic resistance genes (ARGs) in sludge cultured with different concentrations of tetracycline. Pyrosequencing of 16S rRNA gene revealed that tetracycline treatment greatly affected the bacterial community structure of the sludge. Nine genera consisting of Sulfuritalea, Armatimonas, Prosthecobacter, Hyphomicrobium, Azonexus, Longilinea, Paracoccus, Novosphingobium and Rhodobacter were identified as potential TRB in the sludge. Results of qPCR, molecular cloning and metagenomic analysis consistently indicated that tetracycline treatment could increase both the abundance and diversity of the tet genes, but decreased the occurrence and diversity of non-tetracycline ARG, especially sulfonamide resistance gene sul2. Cluster analysis showed that tetracycline treatment at subinhibitory concentrations (5 mg/L) was found to pose greater effects on the bacterial community composition, which may be responsible for the variations of the ARGs abundance. This study indicated that joint use of 454 pyrosequencing and Illumina high-throughput sequencing can be effectively used to explore ARB and ARGs in the environment, and future studies should include an in-depth investigation of the relationship between microbial community, ARGs and antibiotics in sewage treatment plant (STP) sludge.
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