Evidence map›Paper›PMID 24904732›Full record

ArticleInterface focus2014

A computational model for the formation of lamin-B mitotic spindle envelope and matrix.

Changji Shi, Wilbur E Channels, Yixian Zheng, Pablo A Iglesias

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Interface focus, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Mitotic spindle assembly in animal cells: a fine balancing act.Nature reviews. Molecular cell biology · 2017
    Review
  3. Spatial organization of the Ran pathway by microtubules in mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article
  4. Use of Xenopus cell-free extracts to study size regulation of subcellular structures.The International journal of developmental biology · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Changji ShiDepartment of Electrical and Computer Engineering , The Johns Hopkins University , 3400 North Charles Street, Baltimore, MD 21218 , USA.
Wilbur E ChannelsDepartment of Electrical and Computer Engineering , The Johns Hopkins University , 3400 North Charles Street, Baltimore, MD 21218 , USA.
Yixian ZhengDepartment of Embryology , Carnegie Institution of Washington , 3520 San Martin Drive, Baltimore, MD 21218 , USA.
Pablo A IglesiasDepartment of Electrical and Computer Engineering , The Johns Hopkins University , 3400 North Charles Street, Baltimore, MD 21218 , USA.

Funding

The function and assembly of the lamin-B spindle sheathR01GM056312 · NIGMS · CARNEGIE INSTITUTION OF WASHINGTON, D.C. · PI ZHENG, YIXIAN · 1997 to 2014
$2.7M
NIGMS NIH HHS R01 GM056312
6 · The paper itself

Abstract

Recent reports show that, after nuclear envelope breakdown, lamin-B, a component of the nuclear lamina in interphase, localizes around the mitotic spindle as a membranous network. How this process occurs, however, and how it influences mitotic spindle morphogenesis is unclear. Here, we develop a computational model based on a continuum description to represent the abundance and location of various molecular species involved during mitosis, and use the model to test a number of hypotheses regarding the formation of the mitotic matrix. Our model illustrates that freely diffusible nuclear proteins can be captured and transported to the spindle poles by minus-end-directed microtubule (MT) motors. Moreover, simulations show that these proteins can be used to build a shell-like region that envelopes the mitotic spindle, which helps to improve the focusing of the mitotic spindle by spatially restricting MT polymerization and limiting the effective diffusion of the free MTs. Simulations also confirm that spatially dependent regulation of the spindle network through the Ran system improves spindle focusing and morphology. Our results agree with experimental observations that lamin-B reorganizes around the spindle and helps to maintain spindle morphology.

Indexed as

lamin-Bmicrotubulesmitotic matrixmitotic spindleRanspindle envelope

Identifiers

PMID24904732
PMCPMC3996581

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.