ArticleFrontiers in microbiology2014
Connecting lignin-degradation pathway with pre-treatment inhibitor sensitivity of Cupriavidus necator.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Cas3-mediated genome reduction: demonstration in Cupriavidus necator H16 improves growth on heterotrophic and autotrophic carbon sources.Nucleic acids research · 2026Article
- Hydrogen-driven, ATP-dependent biocatalytic reduction of carboxylic acids under non-explosive conditions.Green chemistry : an international journal and green chemistry resource : GC · 2025Article
- Cultivation ofBiotechnology reports (Amsterdam, Netherlands) · 2025Article
- Recent advances in engineering non-native microorganisms for poly(3-hydroxybutyrate) production.World journal of microbiology & biotechnology · 2025Review
- Evaluating lignin degradation under limited oxygen conditions by bacterial isolates from forest soil.Scientific reports · 2024Article
- Carbon dioxide valorization into resveratrol via lithoautotrophic fermentation using engineered Cupriavidus necator H16.Microbial cell factories · 2024Article
- The chemical logic of enzymatic lignin degradation.Chemical communications (Cambridge, England) · 2024Review
- Recent Advancements and Challenges in Lignin Valorization: Green Routes towards Sustainable Bioproducts.Molecules (Basel, Switzerland) · 2022Review
- A Review on EnhancingFrontiers in bioengineering and biotechnology · 2022Review
- OrtSuite: from genomes to prediction of microbial interactions within targeted ecosystem processes.Life science alliance · 2021Article
- Disruption of theFrontiers in microbiology · 2021Article
- Securing a furan-based biorefinery: disclosing the genetic basis of the degradation of hydroxymethylfurfural and its derivatives in the model fungus Aspergillus nidulans.Microbial biotechnology · 2020Article
- A Sustainable Chemicals Manufacturing Paradigm Using COiScience · 2020Article
- Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-AccumulatingFrontiers in microbiology · 2018Article
- Tolerance and metabolic response ofBiotechnology for biofuels · 2018Article
- Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms.Frontiers in bioengineering and biotechnology · 2018Article
- Biodegradation of alkaline lignin byBiotechnology for biofuels · 2017Article
- Zymomonas mobilis as a model system for production of biofuels and biochemicals.Microbial biotechnology · 2016Review
- Lytic polysaccharide monooxygenases from Myceliophthora thermophila C1 differ in substrate preference and reducing agent specificity.Biotechnology for biofuels · 2016Article
- Rice husks and their hydrochars cause unexpected stress response in the nematode Caenorhabditis elegans: reduced transcription of stress-related genes.Environmental science and pollution research international · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To produce lignocellulosic biofuels economically, the complete release of monomers from the plant cell wall components, cellulose, hemicellulose, and lignin, through pre-treatment and hydrolysis (both enzymatic and chemical), and the efficient utilization of these monomers as carbon sources, is crucial. In addition, the identification and development of robust microbial biofuel production strains that can tolerate the toxic compounds generated during pre-treatment and hydrolysis is also essential. In this work, Cupriavidus necator was selected due to its capabilities for utilizing lignin monomers and producing polyhydroxylbutyrate (PHB), a bioplastic as well as an advanced biofuel intermediate. We characterized the growth kinetics of C. necator in pre-treated corn stover slurry as well as individually in the pre-sence of 11 potentially toxic compounds in the saccharified slurry. We found that C. necator was sensitive to the saccharified slurry produced from dilute acid pre-treated corn stover. Five out of 11 compounds within the slurry were characterized as toxic to C. necator, namely ammonium acetate, furfural, hydroxymethylfurfural (HMF), benzoic acid, and p-coumaric acid. Aldehydes (e.g., furfural and HMF) were more toxic than the acetate and the lignin degradation products benzoic acid and p-coumaric acid; furfural was identified as the most toxic compound. Although toxic to C. necator at high concentration, ammonium acetate, benzoic acid, and p-coumaric acid could be utilized by C. necator with a stimulating effect on C. necator growth. Consequently, the lignin degradation pathway of C. necator was reconstructed based on genomic information and literature. The efficient conversion of intermediate catechol to downstream products of cis,cis-muconate or 2-hydroxymuconate-6-semialdehyde may help improve the robustness of C. necator to benzoic acid and p-coumaric acid as well as improve PHB productivity.
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