ArticleGenome research2014
Digital expression profiling of the compartmentalized translatome of Purkinje neurons.
Article in Genome research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
- Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue.Cell reports methods · 2024Article
- Functional and morphological adaptation of medial prefrontal corticotropin releasing factor receptor 1-expressing neurons in male mice following chronic ethanol exposure.Neurobiology of stress · 2024Article
- The brain's dark transcriptome: Sequencing RNA in distal compartments of neurons and glia.Current opinion in neurobiology · 2023Review
- Spatial transcriptomic analysis of Sonic hedgehog medulloblastoma identifies that the loss of heterogeneity and promotion of differentiation underlies the response to CDK4/6 inhibition.Genome medicine · 2023Article
- Article
- Ribosome-Associated ncRNAs (rancRNAs) Adjust Translation and Shape Proteomes.Non-coding RNA · 2022Review
- Chromatin architecture at susceptible gene loci in cerebellar Purkinje cells characterizes DNA damage-induced neurodegeneration.Science advances · 2021Article
- Hippocampal neurons' cytosolic and membrane-bound ribosomal transcript profiles are differentially regulated by learning and subsequent sleep.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Dopamine neuron-specific LRRK2 G2019S effects on gene expression revealed by translatome profiling.Neurobiology of disease · 2021Article
- SCA7 Mouse Cerebellar Pathology Reveals Preferential Downregulation of Key Purkinje Cell-Identity Genes and Shared Disease Signature with SCA1 and SCA2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Neuronal metabolic rewiring promotes resilience to neurodegeneration caused by mitochondrial dysfunction.Science advances · 2020Article
- A Picture Worth a Thousand Molecules-Integrative Technologies for Mapping Subcellular Molecular Organization and Plasticity in Developing Circuits.Frontiers in synaptic neuroscience · 2020Article
- Axon TRAP reveals learning-associated alterations in cortical axonal mRNAs in the lateral amgydala.eLife · 2019Article
- Selective translational usage of TSS and core promoters revealed by translatome sequencing.BMC genomics · 2019Article
- Single-cell nanobiopsy reveals compartmentalization of mRNAs within neuronal cells.The Journal of biological chemistry · 2018Article
- The Expanding Toolkit of Translating Ribosome Affinity Purification.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Linking FANTOM5 CAGE peaks to annotations with CAGEscan.Scientific data · 2017Article
- Transcriptomic Analysis of Ribosome-Bound mRNA in Cortical NeuritesThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Local translation in neuronal compartments: how local is local?EMBO reports · 2017Review
- DEIVA: a web application for interactive visual analysis of differential gene expression profiles.BMC genomics · 2017Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Underlying the complexity of the mammalian brain is its network of neuronal connections, but also the molecular networks of signaling pathways, protein interactions, and regulated gene expression within each individual neuron. The diversity and complexity of the spatially intermingled neurons pose a serious challenge to the identification and quantification of single neuron components. To address this challenge, we present a novel approach for the study of the ribosome-associated transcriptome-the translatome-from selected subcellular domains of specific neurons, and apply it to the Purkinje cells (PCs) in the rat cerebellum. We combined microdissection, translating ribosome affinity purification (TRAP) in nontransgenic animals, and quantitative nanoCAGE sequencing to obtain a snapshot of RNAs bound to cytoplasmic or rough endoplasmic reticulum (rER)-associated ribosomes in the PC and its dendrites. This allowed us to discover novel markers of PCs, to determine structural aspects of genes, to find hitherto uncharacterized transcripts, and to quantify biophysically relevant genes of membrane proteins controlling ion homeostasis and neuronal electrical activities.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.