ArticleCurrent protocols in chemical biology2014
Manipulating natural product biosynthetic pathways via DNA assembler.
Article in Current protocols in chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- A Versatile Enzymatic Pathway for Modification of Peptide C‑Termini.ACS central science · 2025Article
- Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method.Nature protocols · 2024Review
- CRISETR: an efficient technology for multiplexed refactoring of biosynthetic gene clusters.Nucleic acids research · 2024Article
- Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production.Nature communications · 2023Article
- Metabolic engineering of low-pH-tolerant non-model yeast,Metabolic engineering communications · 2023Article
- Exploring the Use of Cold Atmospheric Plasma to Overcome Drug Resistance in Cancer.Biomedicines · 2023Review
- Increasing the heterologous production of spinosad inSynthetic and systems biotechnology · 2021Article
- Review
- Metabolic engineering of an acid-tolerant yeast strainMetabolic engineering communications · 2020Article
- Synthetic Biology Approaches to EngineerLife (Basel, Switzerland) · 2020Review
- Protocols for yTREX/Tn5-based gene cluster expression in Pseudomonas putida.Microbial biotechnology · 2020Article
- Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Heterologous expression-facilitated natural products' discovery in actinomycetes.Journal of industrial microbiology & biotechnology · 2019Review
- Article
- Discovery of microbial natural products by activation of silent biosynthetic gene clusters.Nature reviews. Microbiology · 2015Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
DNA assembler is an efficient synthetic biology method for constructing and manipulating biochemical pathways. The rapidly increasing number of sequenced genomes provides a rich source for discovery of gene clusters involved in synthesizing new natural products. However, both discovery and economical production are hampered by our limited knowledge in manipulating most organisms and the corresponding pathways. By taking advantage of yeast in vivo homologous recombination, DNA assembler synthesizes an entire expression vector containing the target biosynthetic pathway and the genetic elements needed for DNA maintenance and replication. Here we use the spectinabilin clusters originated from two hosts as examples to illustrate the guidelines of using DNA assembler for cluster characterization and silent cluster activation. Such strategies offer unprecedented versatility in cluster manipulation, bypass the traditional laborious strategies to elicit pathway expression, and provide a new platform for de novo cluster assembly and genome mining for discovering new natural products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.