ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2014
Rac1 GTPase silencing counteracts microgravity-induced effects on osteoblastic cells.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Impact of Microgravity on Cytoskeletal Dynamics, Protein Transport, and Signaling Networks: Potential Therapeutic Opportunities for Skin Health.International journal of molecular sciences · 2026Review
- Astroimmunology: the effects of spaceflight and its associated stressors on the immune system.Nature reviews. Immunology · 2026Review
- Single-cell analysis identifies conserved features of immune dysfunction in simulated microgravity and spaceflight.Nature communications · 2024Article
- Omics Studies of Tumor Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Transcriptomics analysis reveals potential mechanisms underlying mitochondrial dysfunction and T cell exhaustion in astronauts' blood cells in space.Frontiers in immunology · 2024Article
- Microgravity triggers ferroptosis and accelerates senescence in the MG-63 cell model of osteoblastic cells.NPJ microgravity · 2023Article
- The role of wnt signaling in diabetes-induced osteoporosis.Diabetology & metabolic syndrome · 2023Review
- A multi-omics longitudinal study of the murine retinal response to chronic low-dose irradiation and simulated microgravity.Scientific reports · 2022Article
- The effects of microgravity on bone structure and function.NPJ microgravity · 2022Review
- Osteocytes and Weightlessness.Current osteoporosis reports · 2021Review
- Butyrate-containing structured lipids inhibit RAC1 and epithelial-to-mesenchymal transition markers: a chemopreventive mechanism against hepatocarcinogenesis.The Journal of nutritional biochemistry · 2020Article
- The Impact of Spaceflight and Simulated Microgravity on Cell Adhesion.International journal of molecular sciences · 2020Review
- Spaceflight-Induced Bone Tissue Changes that Affect Bone Quality and Increase Fracture Risk.Current osteoporosis reports · 2020Review
- Real Microgravity Influences the Cytoskeleton and Focal Adhesions in Human Breast Cancer Cells.International journal of molecular sciences · 2019Article
- Gene Expression in Osteoblasts and Osteoclasts Under Microgravity Conditions: A Systematic Review.Current genomics · 2019Review
- Rapid adaptation to microgravity in mammalian macrophage cells.Scientific reports · 2017Article
- RhoGTPase stimulation is associated with strontium chloride treatment to counter simulated microgravity-induced changes in multipotent cell commitment.NPJ microgravity · 2017Article
- Cytoskeletal stability and metabolic alterations in primary human macrophages in long-term microgravity.PloS one · 2017Article
- ROCK inhibition enhances neurite outgrowth in neural stem cells by upregulating YAP expression in vitro.Neural regeneration research · 2016Article
- RhoGTPases as key players in mammalian cell adaptation to microgravity.BioMed research international · 2015Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone cells exposed to real microgravity display alterations of their cytoskeleton and focal adhesions, two major mechanosensitive structures. These structures are controlled by small GTPases of the Ras homology (Rho) family. We investigated the effects of RhoA, Rac1, and Cdc42 modulation of osteoblastic cells under microgravity conditions. Human MG-63 osteoblast-like cells silenced for RhoGTPases were cultured in the automated Biobox bioreactor (European Space Agency) aboard the Foton M3 satellite and compared to replicate ground-based controls. The cells were fixed after 69 h of microgravity exposure for postflight analysis of focal contacts, F-actin polymerization, vascular endothelial growth factor (VEGF) expression, and matrix targeting. We found that RhoA silencing did not affect sensitivity to microgravity but that Rac1 and, to a lesser extent, Cdc42 abrogation was particularly efficient in counteracting the spaceflight-related reduction of the number of focal contacts [-50% in silenced, scrambled (SiScr) controls vs. -15% for SiRac1], the number of F-actin fibers (-60% in SiScr controls vs. -10% for SiRac1), and the depletion of matrix-bound VEGF (-40% in SiScr controls vs. -8% for SiRac1). Collectively, these data point out the role of the VEGF/Rho GTPase axis in mechanosensing and validate Rac1-mediated signaling pathways as potential targets for counteracting microgravity effects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.