ArticleNature protocols2014
Tobacco rattle virus-based virus-induced gene silencing in Nicotiana benthamiana.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 167 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
167 citing papers in PubMed, 390 citations in OpenAlex.
- Turnip mosaic virus utilizes the lipid droplet biogenesis machinery to facilitate its propagation in plants.The New phytologist · 2026Article
- Transforming Plant Viruses into Vectors for Next-Generation Agriculture-A Review.Current microbiology · 2026Review
- Turnip Mosaic Virus-Based gRNA Delivery System for Plant Genome Editing.Plant direct · 2026Article
- Transgene-free plant genome editing via viral delivery of miniature CRISPR-Cas12f.Functional & integrative genomics · 2026Article
- Plant viruses as next-generation vectors for transgene-free genome editing, gene regulation, and rapid crop improvement.Plant molecular biology · 2026Review
- Pepper G-type Lectin Receptor-like Kinase, CaRLK1, Modulates ABA-Mediated Stomatal Closure and Drought Tolerance.The plant pathology journal · 2026Article
- Tobamoviruses CP Proteins Hijack Light-Induced Protein (NbLIP1) to Promote Viral Replication by Facilitating VRO Formation.Plant biotechnology journal · 2026Article
- Competitive hijacking of fibrillarin by TBTV P3 and TVDV CP mediates a complex synergistic interaction.BMC plant biology · 2026Article
- A dual-substrate methyltransferase catalyzes consecutive methylation reactions to convert proline into stachydrine in Leonurus japonicus.Plant communications · 2026Article
- Dual-Role GH7 Family Proteins From Verticillium dahliae Function as Virulence Factors and Intracellular Effectors Triggering NLR-Mediated Immunity.Plant, cell & environment · 2026Article
- The impact of CsPSKs on plant immunity: insights from a genome-wide study of small peptides in Citrus sinensis.Functional & integrative genomics · 2026Article
- Establishment of a TRV-VIGS system in Coix (Frontiers in plant science · 2026Article
- Protocol for triggering plant gene silencing by viral delivery of short RNA.STAR protocols · 2025Article
- Mitochondrial ROS trigger interorganellular signaling and prime ER processes to establish enhanced plant immunity.Science advances · 2025Article
- Phytophthora Avr3a-Like Effectors Target and Inhibit Cinnamyl Alcohol Dehydrogenase CAD5 to Suppress Plant Immunity.Molecular plant pathology · 2025Article
- Late blight pathogen targets host Rab-G3 GTPases with an atypical GTPase-activating protein.Journal of integrative plant biology · 2025Article
- Nanoparticle Delivery of Antisense miR162 Inhibits Invasive Habitat Adaption of Alternanthera Philoxeroides.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Efficient Virus-Induced Gene Silencing (VIGS) Method for Discovery of Resistance Genes in Soybean.Plants (Basel, Switzerland) · 2025Article
- AmBio · 2025Article
- Heritable virus-induced germline editing in tomato.The Plant journal : for cell and molecular biology · 2025Article
107 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) is widely used in various plant species to downregulate the expression of a target plant gene. TRV is a bipartite, positive-strand RNA virus with the TRV1 and TRV2 genomes. To induce post-transcriptional gene silencing (PTGS), the TRV2 genome is genetically modified to carry a fragment of the target gene and delivered into the plant (along with the TRV1 genome) by agroinoculation. TRV1- and TRV2-carrying Agrobacterium strains are then co-inoculated into 3-week-old plant leaves by one of three methods: a needleless syringe, the agrodrench method or by pricking with a toothpick. Target gene silencing occurs in the newly developed noninoculated leaves within 2-3 weeks of TRV inoculation. The TRV-VIGS protocol described here takes only 4 weeks to implement, and it is faster and easier to perform than other gene silencing techniques that are currently available. Although we use Nicotiana benthamiana as an example, the protocol is adaptable to other plant species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.