ArticleNature protocols2014
Quantification of larval zebrafish motor function in multiwell plates using open-source MATLAB applications.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed.
- Versatile CRISPR-Cas Tools for Gene Regulation in Zebrafish via an Enhanced Q Binary System.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A human Tau expressing zebrafish model of progressive supranuclear palsy identifies Brd4 as a regulator of microglial synaptic elimination.Nature communications · 2024Article
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- Full-field exposure of larval zebrafish to narrow waveband LED light sources at defined power and energy for optogenetic applications.Journal of neuroscience methods · 2024Article
- SAMPL is a high-throughput solution to study unconstrained vertical behavior in small animals.Cell reports · 2023Article
- Scalable Apparatus to Measure Posture and Locomotion (SAMPL): a high-throughput solution to study unconstrained vertical behavior in small animals.bioRxiv : the preprint server for biology · 2023Article
- Quantification of functional recovery in a larval zebrafish model of spinal cord injury.Journal of neuroscience research · 2022Article
- Development of a high-throughput tailored imaging method in zebrafish to understand and treat neuromuscular diseases.Frontiers in molecular neuroscience · 2022Article
- Sinusoidal analysis reveals a non-linear and dopamine-dependent relationship between ambient illumination and motor activity in larval zebrafish.Neuroscience letters · 2021Article
- Zebrafish tracking using YOLOv2 and Kalman filter.Scientific reports · 2021Article
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- α-Synuclein amplifies cytoplasmic peroxide flux and oxidative stress provoked by mitochondrial inhibitors in CNS dopaminergic neurons in vivo.Redox biology · 2020Article
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- A limb-girdle muscular dystrophy 2I model of muscular dystrophy identifies corrective drug compounds for dystroglycanopathies.JCI insight · 2018Article
- High-throughput Screening in Larval Zebrafish Identifies Novel Potent Sedative-hypnotics.Anesthesiology · 2018Article
- Modulation of the zebrafish optokinetic reflex by pharmacologic agents targeting GABANeuroscience letters · 2018Article
- Embryonic exposure to ethanol increases the susceptibility of larval zebrafish to chemically induced seizures.Scientific reports · 2018Article
- An open-source method to analyze optokinetic reflex responses in larval zebrafish.Journal of neuroscience methods · 2018Article
- Automatic multiple zebrafish larvae tracking in unconstrained microscopic video conditions.Scientific reports · 2017Article
- Action sequencing in the spontaneous swimming behavior of zebrafish larvae - implications for drug development.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
This article describes a method to quantify the movements of larval zebrafish in multiwell plates, using the open-source MATLAB applications LSRtrack and LSRanalyze. The protocol comprises four stages: generation of high-quality, flatly illuminated video recordings with exposure settings that facilitate object recognition; analysis of the resulting recordings using tools provided in LSRtrack to optimize tracking accuracy and motion detection; analysis of tracking data using LSRanalyze or custom MATLAB scripts; and implementation of validation controls. The method is reliable, automated and flexible, requires <1 h of hands-on work for completion once optimized and shows excellent signal:noise characteristics. The resulting data can be analyzed to determine the following: positional preference; displacement, velocity and acceleration; and duration and frequency of movement events and rest periods. This approach is widely applicable to the analysis of spontaneous or stimulus-evoked zebrafish larval neurobehavioral phenotypes resulting from a broad array of genetic and environmental manipulations, in a multiwell plate format suitable for high-throughput applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.