ArticleBiochemistry2014
Following drug uptake and reactions inside Escherichia coli cells by Raman microspectroscopy.
Article in Biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 33 citations in OpenAlex.
- Classification and prediction ofPeerJ · 2023Article
- An Outer Membrane Vesicle-Based Permeation Assay (OMPA) for Assessing Bacterial Bioavailability.Advanced healthcare materials · 2022Article
- Nanotargeting of Resistant Infections with a Special Emphasis on the Biofilm Landscape.Bioconjugate chemistry · 2021Review
- Biochemical characterization of pathogenic bacterial species using Raman spectroscopy and discrimination model based on selected spectral features.Lasers in medical science · 2021Article
- Defining new chemical space for drug penetration into Gram-negative bacteria.Nature chemical biology · 2020Review
- Stability of β-lactam antibiotics in bacterial growth media.PloS one · 2020Article
- Membrane damage mechanism contributes to inhibition of trans-cinnamaldehyde on Penicillium italicum using Surface-Enhanced Raman Spectroscopy (SERS).Scientific reports · 2019Article
- Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy.Chemical science · 2017Article
- Stimulated Raman scattering microscopy: an emerging tool for drug discovery.Chemical Society reviews · 2016Review
- Exposing a β-Lactamase "Twist": the Mechanistic Basis for the High Level of Ceftazidime Resistance in the C69F Variant of the Burkholderia pseudomallei PenI β-Lactamase.Antimicrobial agents and chemotherapy · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Raman microspectroscopy combined with Raman difference spectroscopy reveals the details of chemical reactions within bacterial cells. The method provides direct quantitative data on penetration of druglike molecules into Escherichia coli cells in situ along with the details of drug-target reactions. With this label-free technique, clavulanic acid and tazobactam can be observed as they penetrate into E. coli cells and subsequently inhibit β-lactamase enzymes produced within these cells. When E. coli cells contain a β-lactamase that forms a stable complex with an inhibitor, the Raman signature of the known enamine acyl-enzyme complex is detected. From Raman intensities it is facile to measure semiquantitatively the number of clavulanic acid molecules taken up by the lactamase-free cells during growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.