Evidence map›Paper›PMID 24901045›Full record

ArticleCell death & disease2014

Retinoblastoma protein (RB) interacts with E2F3 to control terminal differentiation of Sertoli cells.

E Rotgers, A Rivero-Müller, M Nurmio, M Parvinen, F Guillou, I Huhtaniemi, N Kotaja, S Bourguiba-Hachemi, J Toppari

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death & disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.2field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Green tea EGCG inhibits naïve CD4Current research in food science · 2023
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  3. Review
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  9. Article
  10. E2F3 transcription factor: A promising biomarker in lung cancer.Cancer biomarkers : section A of Disease markers · 2017
    Article
  11. Article
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

E Rotgers1] Department of Physiology, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland [2] Department of Pediatrics, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland.
A Rivero-MüllerDepartment of Physiology, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland.
M Nurmio1] Department of Physiology, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland [2] Department of Pediatrics, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland.
M ParvinenDepartment of Physiology, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland.
F GuillouINRA-CNRS-Haras Nationaux-Université de Tours Joint Research Unit for Reproductive and Behavioural Physiology, Tours Research Centre, Nouzilly 3780, France.
I Huhtaniemi1] Department of Physiology, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland [2] Department of Surgery and Cancer, Imperial College London, Hammersmith Campus, London W12 0NN, UK.
N KotajaDepartment of Physiology, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland.
S Bourguiba-Hachemi1] Department of Physiology, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland [2] Biotechnology Department, College of Graduate Studies, Arabian Gulf University, PO BOX 26670 Manama, Bahrain.
J Toppari1] Department of Physiology, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland [2] Department of Pediatrics, University of Turku, Kiinamyllynkatu 10, Turku FIN-20520, Finland.
University of Turku · FICentre National de la Recherche Scientifique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The retinoblastoma protein (RB) is essential for normal cell cycle control. RB function depends, at least in part, on interactions with the E2F family of DNA-binding transcription factors (E2Fs). To study the role of RB in the adult testis, a Sertoli cell (SC)-specific Rb knockout mouse line (SC-RbKO) was generated using the Cre/loxP recombination system. SC-RbKO mice exhibited an age-dependent testicular atrophy, impaired fertility, severe SC dysfunction, and spermatogenic defects. Removal of Rb in SC induced aberrant SC cycling, dedifferentiation, and apoptosis. Here we show that E2F3 is the only E2F expressed in mouse SCs and that RB interacts with E2F3 during mouse testicular development. In the absence of RB, the other retinoblastoma family members p107 and p130 began interacting with E2F3 in the adult testes. In vivo silencing of E2F3 partially restored the SC maturation and survival as well as spermatogenesis in the SC-RbKO mice. These results point to RB as a key regulator of SC function in adult mice and that the RB/E2F3 pathway directs SC maturation, cell cycle quiescence, and RB protects SC from apoptosis.

Indexed as

AnimalsApoptosisCell DifferentiationE2F3 Transcription FactorGene SilencingMaleMiceMice, KnockoutRetinoblastoma ProteinSertoli CellsE2f3 protein, mouseE2F3 Transcription FactorRetinoblastoma Protein

Identifiers

PMID24901045
PMCPMC4611710
OpenAlexW2003612625

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.