ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
Genetically targeted binary labeling of retinal neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 81 citations in OpenAlex.
- Retinal VIP-amacrine cells: their development, structure, and function.Eye (London, England) · 2024Pooled it
- The extreme diversity of retinal amacrine cells has deep evolutionary roots.Science advances · 2026Article
- The Extreme Diversity Of Retinal Amacrine Cells Has Deep Evolutionary Roots.bioRxiv : the preprint server for biology · 2026Article
- The mysterious middlemen making your vision pop: understanding the function of amacrine cells.The Journal of physiology · 2025Review
- Neuroprotective Effect of Methylene Blue in a Rat Model of Traumatic Optic Neuropathy.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Functionally distinct GABAergic amacrine cell types regulate spatiotemporal encoding in the mouse retina.Nature neuroscience · 2025Article
- Retinoic Acid-Dependent Loss of Synaptic Output from Bipolar Cells Impairs Visual Information Processing in Inherited Retinal Degeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- A pupillary contrast response in mice and humans: Neural mechanisms and visual functions.Neuron · 2024Article
- Layers of inhibitory networks shape receptive field properties of AII amacrine cells.Cell reports · 2023Article
- Spatial organization of the mouse retina at single cell resolution by MERFISH.Nature communications · 2023Article
- Functional properties of GABAFrontiers in ophthalmology · 2023Article
- Axonal architecture of the mouse inner retina revealed by second harmonic generation.PNAS nexus · 2022Article
- Intersectional mapping of multi-transmitter neurons and other cell types in the brain.Cell reports · 2022Article
- Upper motor neurons are a target for gene therapy and UCHL1 is necessary and sufficient to improve cellular integrity of diseased upper motor neurons.Gene therapy · 2022Article
- The Slack Channel Deletion Causes Mechanical Pain Hypersensitivity in Mice.Frontiers in molecular neuroscience · 2022Article
- Identification of retinal ganglion cell types and brain nuclei expressing the transcription factor Brn3c/Pou4f3 using a Cre recombinase knock-in allele.The Journal of comparative neurology · 2021Article
- Photoreceptive Ganglion Cells Drive Circuits for Local Inhibition in the Mouse Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Transpupillary Two-photon In vivo Imaging of the Mouse Retina.Journal of visualized experiments : JoVE · 2021Article
- Gbx2 Identifies Two Amacrine Cell Subtypes with Distinct Molecular, Morphological, and Physiological Properties.Cell reports · 2020Article
- Quantification of Adeno-Associated Virus with Safe Nucleic Acid Dyes.Human gene therapy · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
A major stumbling block to understanding neural circuits is the extreme anatomical and functional diversity of interneurons. Subsets of interneurons can be targeted for manipulation using Cre mouse lines, but Cre expression is rarely confined to a single interneuron type. It is essential to have a strategy that further restricts labeling in Cre driver lines. We now describe an approach that combines Cre driver mice, recombinant adeno-associated virus, and rabies virus to produce sparse but binary labeling of select interneurons--frequently only a single cell in a large region. We used this approach to characterize the retinal amacrine and ganglion cell types in five GABAergic Cre mouse (Mus musculus) lines, and identified two new amacrine cell types: an asymmetric medium-field type and a wide-field type. We also labeled several wide-field amacrine cell types that have been previously identified based on morphology but whose connectivity and function had not been systematically studied due to lack of genetic markers. All Cre-expressing amacrine cells labeled with an antibody to GABA. Cre-expressing RGCs lacked GABA labeling and included classically defined as well as recently identified types. In addition to the retina, our technique leads to sparse labeling of neurons in the cortex, lateral geniculate nucleus, and superior colliculus, and can be used to express optogenetic tools such as channelrhodopsin and protein sensors such as GCaMP. The Cre drivers identified in this study provide genetic access to otherwise hard to access cell types for systematic analysis including anatomical characterization, physiological recording, optogenetic and/or chemical manipulation, and circuit mapping.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.