ArticleScientific reports2014
ATP binding turns plant cryptochrome into an efficient natural photoswitch.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 94 citations in OpenAlex.
- Time-Resolved Native Mass Spectrometry Reveals Reversible Light-Driven Oligomerization of Arabidopsis Cryptochrome 1 and Its Antagonism by BIC1.Angewandte Chemie (International ed. in English) · 2026Article
- Decrypting the Nonadiabatic Photoinduced Electron Transfer Mechanism in Light-Sensing Cryptochrome.ACS central science · 2025Article
- Photochemistry of Receptor-Bound Flavin Resolved in Living Human Cells by Infrared Spectroscopy.Journal of the American Chemical Society · 2025Article
- Article
- Balance between photoreduction efficiency, cofactor affinity, and allosteric coupling of halogenase flavoenzymes.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- Importance of Polarizable Embedding for Absorption Spectrum Calculations ofThe journal of physical chemistry. B · 2024Article
- Understanding the Red Shift in the Absorption Spectrum of the FAD Cofactor in ClCry4 Protein.The journal of physical chemistry. B · 2024Article
- A structural decryption of cryptochromes.Frontiers in chemistry · 2024Review
- 'Seeing' the electromagnetic spectrum: spotlight on the cryptochrome photocycle.Frontiers in plant science · 2024Review
- Cryptochrome and quantum biology: unraveling the mysteries of plant magnetoreception.Frontiers in plant science · 2023Review
- Unraveling the Photoactivation Mechanism of a Light-Activated Adenylyl Cyclase Using Ultrafast Spectroscopy Coupled with Unnatural Amino Acid Mutagenesis.ACS chemical biology · 2022Article
- Limited solvation of an electron donating tryptophan stabilizes a photoinduced charge-separated state in plant (6-4) photolyase.Scientific reports · 2022Article
- Open hardware microsecond dispersive transient absorption spectrometer for linear optical response.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Article
- Cryptochrome magnetoreception: four tryptophans could be better than three.Journal of the Royal Society, Interface · 2021Article
- Cryptochromes: Photochemical and structural insight into magnetoreception.Protein science : a publication of the Protein Society · 2021Review
- Investigation of the pH-dependence of the oxidation of FAD in VcCRY-1, a member of the cryptochrome-DASH family.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021Article
- Ultrafast photoreduction dynamics of a new class of CPD photolyases.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021Article
- Does Green Really Mean Go? Increasing the Fraction of Green Photons Promotes Growth of Tomato but Not Lettuce or Cucumber.Plants (Basel, Switzerland) · 2021Article
- Identification of the vibrational marker of tyrosine cation radical using ultrafast transient infrared spectroscopy of flavoprotein systems.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021Article
- Tuning flavin environment to detect and control light-induced conformational switching in Drosophila cryptochrome.Communications biology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptochromes are flavoproteins that drive diverse developmental light-responses in plants and participate in the circadian clock in animals. Plant cryptochromes have found application as photoswitches in optogenetics. We have studied effects of pH and ATP on the functionally relevant photoreduction of the oxidized FAD cofactor to the semi-reduced FADH(·) radical in isolated Arabidopsis cryptochrome 1 by transient absorption spectroscopy on nanosecond to millisecond timescales. In the absence of ATP, the yield of light-induced radicals strongly decreased with increasing pH from 6.5 to 8.5. With ATP present, these yields were significantly higher and virtually pH-independent up to pH 9. Analysis of our data in light of the crystallographic structure suggests that ATP-binding shifts the pKa of aspartic acid D396, the putative proton donor to FAD·(-), from ~7.4 to >9, and favours a reaction pathway yielding long-lived aspartate D396(-). Its negative charge could trigger conformational changes necessary for signal transduction.
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