ArticleNature communications2014
Hybrid multiphoton volumetric functional imaging of large-scale bioengineered neuronal networks.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
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- Viscocohesive hyaluronan gel enhances stability of intravital multiphoton imaging with subcellular resolution.Neurophotonics · 2025Article
- Multifocal microscopy for functional imaging of neural systems.Neurophotonics · 2024Article
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- Multiline orthogonal scanning temporal focusing (mosTF) microscopy for scattering reduction in in vivo brain imaging.Scientific reports · 2024Article
- Multiline Orthogonal Scanning Temporal Focusing (mosTF) Microscopy for Scattering Reduction in High-speedResearch square · 2023Article
- Optical gearbox enabled versatile multiscale high-throughput multiphoton functional imaging.Nature communications · 2022Article
- Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.Micromachines · 2021Review
- Human mini-brain models.Nature biomedical engineering · 2021Review
- Temporal focusing multiphoton microscopy with optimized parallel multiline scanning for fast biotissue imaging.Journal of biomedical optics · 2021Article
- Recent progress in translational engineered in vitro models of the central nervous system.Brain : a journal of neurology · 2020Review
- Innovations in 3-Dimensional Tissue Models of Human Brain Physiology and Diseases.Advanced functional materials · 2020Article
- Functional Characterization of Three-Dimensional Cortical Cultures for In Vitro Modeling of Brain Networks.iScience · 2020Article
- Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators.Neurophotonics · 2020Article
- Scanless two-photon excitation with temporal focusing.Nature methods · 2020Review
- Accelerated Stimulated Raman Projection Tomography by Sparse Reconstruction From Sparse-View Data.IEEE transactions on bio-medical engineering · 2020Article
- Photobleaching Imprinting Enhanced Background Rejection in Line-Scanning Temporal Focusing Microscopy.Frontiers in chemistry · 2020Article
- Increasing the penetration depth of temporal focusing multiphoton microscopy for neurobiological applications.Journal of physics D: Applied physics · 2019Article
- Volumetric CaCell · 2019Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Planar neural networks and interfaces serve as versatile in vitro models of central nervous system physiology, but adaptations of related methods to three dimensions (3D) have met with limited success. Here, we demonstrate for the first time volumetric functional imaging in a bioengineered neural tissue growing in a transparent hydrogel with cortical cellular and synaptic densities, by introducing complementary new developments in nonlinear microscopy and neural tissue engineering. Our system uses a novel hybrid multiphoton microscope design combining a 3D scanning-line temporal-focusing subsystem and a conventional laser-scanning multiphoton microscope to provide functional and structural volumetric imaging capabilities: dense microscopic 3D sampling at tens of volumes per second of structures with mm-scale dimensions containing a network of over 1,000 developing cells with complex spontaneous activity patterns. These developments open new opportunities for large-scale neuronal interfacing and for applications of 3D engineered networks ranging from basic neuroscience to the screening of neuroactive substances.
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Registered trials
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